Caron A, Malfatti E, Aguejouf O, Faivre-Fiorina B, Menu P
Department of Hematology and Physiology, Faculty of Pharmacy, University Henri Poincaré-Nancy 1, France.
Artif Cells Blood Substit Immobil Biotechnol. 2001 Jan;29(1):19-30. doi: 10.1081/bio-100001253.
Infusion of hemoglobin-based oxygen-carrying solutions (HBOCs) produce an immediate rise in blood pressure with most solutions, both in animals and humans, as a result of systemic and pulmonary vasoconstriction. Autoregulation of the O2 supply by the microvasculature has been proposed as a phenomenon involved in the vasoconstriction elicited by HBOCs. Nevertheless, little is known about the ability of various HBOCs to induce constriction in the microcirculation according to their specific physicochemical properties (viscosity, molecular weight, P50, etc.). This study was therefore designed to assess the effects of three HBOCs, that is, bis(3.5-dibromosalicyl) fumarate-crosslinked hemoglobin (alphaalpha-Hb), dextran-benzene-tetracarboxylate-conjugated hemoglobin (Hb-Dex-BTC) and o-raffinose-oligomerized hemoglobin (o-raffinose-Hb), on the vascular tone of rat mesenteric arterioles (diameter, 15-25 microm) viewed microscopically in moderate hemodilution conditions. The effects of HBOCs were compared to those elicited by a reference solution of hydroxyethyl starch (HES-200) infused in the same conditions. In each experimental group, a fall in arteriolar diameter was observed 2 min and 5 min after infusion of the solution. The maximum changes were observed in Hb-Dex-BTC and o-raffinose-Hb groups, in which diameter decreased from 6.9 +/- 0.5% and 5.2 +/- 0.7%, respectively, 2 min after infusion. The changes in arteriolar diameter induced by Hb-Dex-BTC and o-raffinose-Hb were significantly higher than those elicited by HES-200 and alphaalpha-Hb. In conclusion, our data indicate that moderate hemodilution with HBOCs induces instantaneous constriction in rat mesenteric arterioles, with amplitudes depending on both pharmacological and physicochemical properties of the hemoglobin solution infused.
输注基于血红蛋白的携氧溶液（HBOCs）会使大多数溶液在动物和人类体内引起血压立即升高，这是全身和肺血管收缩的结果。微血管对氧气供应的自动调节被认为是HBOCs引发血管收缩所涉及的一种现象。然而，对于各种HBOCs根据其特定物理化学性质（粘度、分子量、P50等）在微循环中诱导收缩的能力知之甚少。因此，本研究旨在评估三种HBOCs，即双（3,5-二溴水杨酸）富马酸交联血红蛋白（αα-Hb）、葡聚糖-苯四羧酸共轭血红蛋白（Hb-Dex-BTC）和邻棉子糖寡聚血红蛋白（邻棉子糖-Hb），在中度血液稀释条件下对大鼠肠系膜小动脉（直径15 - 25微米）血管张力的影响。将HBOCs的作用与在相同条件下输注的羟乙基淀粉（HES - 200）参考溶液所引发的作用进行比较。在每个实验组中，输注溶液后2分钟和5分钟观察到小动脉直径下降。在Hb-Dex-BTC组和邻棉子糖-Hb组中观察到最大变化，输注后2分钟直径分别下降6.9±0.5%和5.2±0.7%。Hb-Dex-BTC和邻棉子糖-Hb诱导的小动脉直径变化显著高于HES - 200和αα-Hb所引发的变化。总之，我们的数据表明，用HBOCs进行中度血液稀释会在大鼠肠系膜小动脉中引起瞬时收缩，收缩幅度取决于所输注血红蛋白溶液的药理和物理化学性质。
