Ramos Y F, Stad R, Attema J, Peltenburg L T, van der Eb A J, Jochemsen A G
Department of Molecular Cell Biology and Center of Biomedical Genetics, Leiden University Medical Center, The Netherlands.
Cancer Res. 2001 Mar 1;61(5):1839-42.
It has been shown that the Hdmx gene is amplified in a subset of gliomas, but thus far, no data are available on HDMX protein expression in tumor cells. We now report that a significant fraction of tumor cell lines expresses increased HDMX levels compared with normal cells; in general, HDMX expression in these tumor cell lines correlates with the presence of wild-type p53. Analysis of tumor material showed that high HDMX expression is not a result of cell line establishment. Interestingly, several cell lines express alternative, shorter HDMX proteins. These results suggest that deregulated expression of HDMX plays a role in carcinogenesis as an alternative way to inactivate p53.
已有研究表明，Hdmx基因在部分胶质瘤中发生扩增，但到目前为止，尚无关于肿瘤细胞中HDMX蛋白表达的数据。我们现在报告，与正常细胞相比，相当一部分肿瘤细胞系中HDMX水平升高；一般来说，这些肿瘤细胞系中的HDMX表达与野生型p53的存在相关。对肿瘤材料的分析表明，HDMX的高表达不是细胞系建立的结果。有趣的是，几个细胞系表达了其他较短的HDMX蛋白。这些结果表明，HDMX的失调表达作为使p53失活的另一种方式，在致癌过程中发挥作用。
