Fujimaki K, Maruta A, Yoshida M, Yamazaki E, Motomura S, Kodama F, Matsuzaki M, Fujisawa S, Kanamori H, Ishigatsubo Y
Department of Hematology, Kanagawa Cancer Center.
Rinsho Ketsueki. 2001 Feb;42(2):89-93.
In patients with both p210-bcr-abl (p210) and p190-bcr-abl (p190)-positive acute lymphoblastic leukemia, the number of p190 transcripts is lower than that of p210 transcripts. It is speculated that the p190 transcript occurs as a consequence of alternative splicing or missplicing events in the BCR gene. Four patients with both p210- and p190-positive acute lymphoblastic leukemia were studied for expression of p210 and p190 by RT-PCR before and after allogeneic bone marrow transplantation. p190 negativity was documented in all four patients, followed by p210 negativity one to two months later in three patients. These results suggest that negativity for p190 indicates an ongoing decrease in the small number of residual leukemic cells. In one patient p190 appeared transiently in spite of prolonged negativity for p210 18 months after bone marrow transplantation. We conclude that analysis of p210 and p190 is useful for following up patients with both p210- and p190-positive acute lymphoblastic leukemia.
在同时存在p210-bcr-abl（p210）和p190-bcr-abl（p190）阳性的急性淋巴细胞白血病患者中，p190转录本的数量低于p210转录本。据推测，p190转录本是BCR基因中选择性剪接或错配剪接事件的结果。对4例p210和p190均阳性的急性淋巴细胞白血病患者在异基因骨髓移植前后通过逆转录聚合酶链反应（RT-PCR）研究p210和p190的表达。所有4例患者均记录到p190阴性，3例患者在1至2个月后出现p210阴性。这些结果表明，p190阴性表明残留的少量白血病细胞数量持续减少。1例患者在骨髓移植18个月后，尽管p210长期阴性，但p190短暂出现。我们得出结论，对p210和p190的分析有助于对p210和p190均阳性的急性淋巴细胞白血病患者进行随访。
