Chang M, Shi Y, Nail S L, HogenEsch H, Adams S B, White J L, Hem S L
Department of Industrial and Physical Pharmacy, Purdue University, West Lafayette, IN 47907, USA.
Vaccine. 2001 Apr 6;19(20-22):2884-9. doi: 10.1016/s0264-410x(00)00559-4.
The effect of the degree of adsorption of lysozyme by aluminium hydroxide adjuvant on the immune response in rabbits was studied. The surface charge of the adjuvant was modified by pretreatment with phosphate anion to produce five vaccines having degrees of adsorption ranging from 3 to 90%. The degree of adsorption of vaccines exhibiting 3, 35 or 85% adsorption changed to 40% within 1 h after each vaccine was mixed with sheep interstitial fluid to simulate subcutaneous administration. The mean anti-lysozyme antibody titers produced by the vaccines were the same and were four times greater than that produced by a lysozyme solution. Thus, the degree of adsorption of lysozyme in sheep interstitial fluid rather than the degree of adsorption in the vaccine correlated with the immune response.
研究了氢氧化铝佐剂对溶菌酶的吸附程度对家兔免疫反应的影响。通过用磷酸根阴离子预处理来改变佐剂的表面电荷，从而制备出五种吸附程度从3%到90%不等的疫苗。将每种疫苗与绵羊间质液混合以模拟皮下给药后，吸附程度为3%、35%或85%的疫苗在1小时内吸附程度均变为40%。这些疫苗产生的平均抗溶菌酶抗体滴度相同，且比溶菌酶溶液产生的抗体滴度高四倍。因此，溶菌酶在绵羊间质液中的吸附程度而非疫苗中的吸附程度与免疫反应相关。
