Kusaba S, Honda C, Kano-Murakami Y
Department of Pomology, National Institute of Fruit Tree Science, 2-1 Fujimoto, Tsukuba, Ibaraki 305-8605, Japan.
J Exp Bot. 2001 Feb;52(355):375-6.
To characterize the gibberellin (GA) 20-oxidase gene in apple, the genomic and cDNA clone from "Fuji" apple (accession no. AB037114) was isolated. The deduced amino acid sequence of this cDNA showed 71% and 66% identity to those of GA 20-oxidase cloned from French bean and Arabidopsis, respectively. The transcript of this gene was detected mainly in immature seeds between 1-3 months after full bloom. These results suggested that this apple GA 20-oxidase gene might be involved in GA biosynthesis in developing apple seed.
