Chernaenko V M, Bresler S E
Mol Biol. 1975 Jan;8(4):467-74.
The interaction of the oligonucleotides ApApA, ApApU and ApApC with polyribouridylic acid and of hexariboinosinic acid with and without phosphate near the 3'-end with polyribocytidylic acid was studied by the method of equilibrium gel filtration through Sephadexes. The free energy, the energy and entropy of the complexing were calculated from isotherms of the adsorption of the oligonucleotides on the polynucleotides in relation to the composition of the oligonucleotides and the concentration of magnesium ions in the buffer. It was shown that in the case of the interaction of ApApA and ApApC with poly(U) a perfect triple complex is formed, while in the case of the interaction of ApApU with poly(U) the noncomplementary base is partially displaced from the complex. The free energy of the interaction of neighboring oligonucleotides in a complex with polynucleotides is from --1000 to --3000 cal/mole depending on the type of the complex. It was shown that after the interaction of hexainosinic acid with poly(C) a double complex is formed. The free energy of the interaction of neighboring oligonucleotides in this complex is formed. The free energy of the interaction of neighboring oligonucleotides in this complex is about --1200 cal/mole. Magnesium ions have a different effect on the formation of triple and double complexes in the interaction of oligonucleotides with polynucleotides.
通过葡聚糖凝胶平衡凝胶过滤法研究了寡核苷酸ApApA、ApApU和ApApC与聚核糖核苷酸的相互作用，以及3'-端附近有或无磷酸的六核糖肌苷酸与聚核糖胞苷酸的相互作用。根据寡核苷酸在多核苷酸上的吸附等温线，结合寡核苷酸的组成和缓冲液中镁离子的浓度，计算了络合的自由能、能量和熵。结果表明，在ApApA和ApApC与聚（U）相互作用的情况下，形成了完美的三元复合物，而在ApApU与聚（U）相互作用的情况下，非互补碱基部分地从复合物中被取代。根据复合物的类型，复合物中相邻寡核苷酸与多核苷酸相互作用的自由能为-1000至-3000卡/摩尔。结果表明，六肌苷酸与聚（C）相互作用后形成了二元复合物。该复合物中相邻寡核苷酸相互作用的自由能约为-1200卡/摩尔。镁离子对寡核苷酸与多核苷酸相互作用中三元和二元复合物的形成有不同的影响。
