Jurowski P, Goś R
Z Kliniki Okulistycznej Instytutu Chirurgii WAM w Łodzi.
Klin Oczna. 2000;102(5):313-7.
Using a direct method, with selective and nitric oxide sensitive electrode Iso-NO, we estimated the level of the Nitric Oxide (NO) in the aqueous humor of the rabbit's eye after phacoemulsification and acrylic foldable lens implantation. We also analysed the NO level during early postoperative days between the 1st and the 5th postoperative day. The current methods of the NO level estimations are also considered.
We examined 30 gray rabbits (weighing 3.0-3.5 kg). Just before surgery, samples of aqueous humor were aspirated. Lens was extracted with phacoemulsification technique. In 15 eyes acrylic foldable IOL was implanted and 15 eyes were left aphacic. The aqueous samples were collected 1, 3, 5 days after surgery. Nitric oxide in each sample was determined with direct, selective nitric oxide sensitive electrode Iso-NO. Because of changing values of the NO concentration and its characteristic course, we expressed the concentration of NO in aqueous humor using field-under-curve method to compare it with control group. The results of our examination are presented in the absolute values.
During physiological conditions the mean value of the field under curve of the NO concentration was estimated as 4987.29 +/- 895.89. After phacoemulsification with or without artificial lens implantation the level of the NO was higher as compared to the control group. After phacoemulsification and acrylic foldable lens implantation the highest level of NO was estimated on the 1st postoperative day as 6917.10 +/- 1199.72. During the examinations, the field under curve of NO concentration was estimated as 6246.63 +/- 327.02 and 5172.28 +/- 552.67 (on the 3rd and 5th days respectively). Only on the 1st and the 3rd postoperative day, the value of the field under curve of NO was significantly higher as compared with the control group. In contrast, after phacoemulsification without lens implantation the highest value of NO field under curve, was estimated on the 3rd postoperative days as 5801.44 +/- 344.45. During the examinations, the field under curve of NO concentration was estimated as 5115.30 +/- 796.11 and 4837.12 +/- 205.87 (on the 1st and the 5th days, respectively). Moreover, it was the only one day of examination with significantly higher value of NO field under curve as compared with the control.
We came to conclusion that the level of nitric oxide in aqueous humor after phacoemulsification with or without artificial lens implantation is higher than in control group. The highest value of the field under curve was estimated on the 1st postoperative day after phacoemulsification and acrylic foldable lens implantation and on the 3rd postoperative day after phacoemulsification without lens implantation.
采用直接法，使用选择性一氧化氮敏感电极Iso-NO，我们评估了白内障超声乳化吸除术联合丙烯酸可折叠人工晶状体植入术后兔眼房水中一氧化氮（NO）的水平。我们还分析了术后第1天至第5天早期术后的NO水平。同时也考虑了当前评估NO水平的方法。
我们检查了30只灰色兔子（体重3.0 - 3.5千克）。手术前，抽取房水样本。采用超声乳化技术摘除晶状体。15只眼植入丙烯酸可折叠人工晶状体，15只眼保持无晶状体状态。术后第1、3、5天收集房水样本。使用直接的选择性一氧化氮敏感电极Iso-NO测定每个样本中的一氧化氮。由于NO浓度值的变化及其特征曲线，我们采用曲线下面积法表示房水中NO的浓度，以便与对照组进行比较。我们检查的结果以绝对值呈现。
在生理条件下，NO浓度曲线下面积的平均值估计为4987.29±895.89。白内障超声乳化吸除术无论是否植入人工晶状体后，NO水平均高于对照组。白内障超声乳化吸除术联合丙烯酸可折叠人工晶状体植入术后，NO的最高水平估计在术后第1天为(6917.10±1199.72)。在检查期间，NO浓度曲线下面积估计为(6246.63±327.02)和(5172.28±552.67)（分别在第3天和第5天）。仅在术后第1天和第3天时，NO曲线下面积的值与对照组相比显著更高。相比之下，白内障超声乳化吸除术未植入晶状体后，NO曲线下面积的最高值估计在术后第3天为(5801.44±344.45)。在检查期间（分别在第1天和第5天），NO浓度曲线下面积估计为(5115.30±796.11)和(4837.12±205.87)。此外，这是唯一一天检查时，NO曲线下面积的值与对照组相比显著更高。
我们得出结论，白内障超声乳化吸除术无论是否植入人工晶状体后房水中一氧化氮水平均高于对照组。白内障超声乳化吸除术联合丙烯酸可折叠人工晶状体植入术后曲线下面积的最高值估计在术后第1天，白内障超声乳化吸除术未植入晶状体后曲线下面积的最高值估计在术后第3天。
