从门多勒（黄斑海鲷）鱼卵中分离并鉴定一种与DNA结合、抑制其复制及其酸沉淀的蛋白质。

Isolation and characterization of a protein from Mendole (Spicara maena) eggs that binds to DNA and inhibits its replication as well as its acid precipitation.

作者信息

Kouretas D, Mamuris Z, Stamatis C, Rodriguez-Burgos A, Tsamis V, Neofitou C

机构信息

School of Agriculture, University of Thessaly, Pedion Areos, Volos, Greece.

出版信息

In Vivo. 2001 Jan-Feb;15(1):49-52.

PMID:11286129

Abstract

Proteins that bind and protect nucleic acids from acid precipitation have been characterized from human and mouse plasma. In the present study, one protein from Mendole (Spicara maena) eggs was purified to homogeneity, by means of acetone fractionation and Sephadex G-100 gel filtration. The protein inhibited DNA replication, exerted by various DNA polymerases. Amino-acid sequence analysis in the amino terminus revealed a unique sequence. Its possible physiological role is discussed.

摘要

已从人和小鼠血浆中鉴定出能结合并保护核酸免受酸沉淀影响的蛋白质。在本研究中，通过丙酮分级分离和葡聚糖凝胶G - 100凝胶过滤，从门多勒（Spicara maena）鱼卵中纯化出一种蛋白质，使其达到同质。该蛋白质可抑制多种DNA聚合酶所引发的DNA复制。对其氨基末端的氨基酸序列分析揭示了一个独特的序列。文中讨论了其可能的生理作用。