Guo J, Ma X Y, Zeng J P
157th Hospital of PLA, Guangzhou Guangdong, P. R. China 510510.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2001 Mar;15(2):106-8.
To study the nerve growth factor (NGF) expression and the influence of IL-1 alpha or IL-1 beta on NGF secretion in newborn rat astrocytes.
Astrocytes obtained from the brain cortex of newborn rats were cultured and purified, and they were divided into three groups, experimental, control and blank groups. IL-1 alpha or IL-1 beta were added into the experimental group with 25, 50 and 100 U/ml, each group was cultured for 24, 48 or 72 hours, and then the NGF contents in cultured astrocytes suspension media were measured by a two-cite enzymelinked immunoserbent assay (ELISA).
Astrocytes could secret NGF by themselves and each concentration of IL-1 alpha or IL-1 beta media at any testing time could enhance NGF secreting in newborn rat astrocytes in certain degrees. The effects of IL-1 beta were stronger than IL-1 alpha, the strongest effect in the unit time was observed in IL-1 beta with 50 U/ml for 24 hours.
Astrocytes can express NGF, and IL-1 alpha or IL-1 beta can enhance the NGF expression in newborn rat astrocytes.
研究新生大鼠星形胶质细胞中神经生长因子（NGF）的表达以及白细胞介素-1α（IL-1α）或白细胞介素-1β（IL-1β）对NGF分泌的影响。
从新生大鼠大脑皮质获取星形胶质细胞并进行培养和纯化，将其分为三组，即实验组、对照组和空白组。向实验组分别加入25、50和100 U/ml的IL-1α或IL-1β，每组培养24、48或72小时，然后用双抗体夹心酶联免疫吸附测定法（ELISA）检测培养的星形胶质细胞悬浮培养基中的NGF含量。
星形胶质细胞自身可分泌NGF，在任何检测时间，各浓度的IL-1α或IL-1β培养基均可在一定程度上增强新生大鼠星形胶质细胞中NGF的分泌。IL-1β的作用强于IL-1α，单位时间内最强作用出现在50 U/ml的IL-1β作用24小时时。
星形胶质细胞可表达NGF，IL-1α或IL-1β可增强新生大鼠星形胶质细胞中NGF的表达。
