Duan Z, Stamatoyannopoulos G, Li Q
Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, Washington 98195, USA.
Mol Cell Biol. 2001 May;21(9):3083-95. doi: 10.1128/MCB.21.9.3083-3095.2001.
The duplicated CCAAT box is required for gamma gene expression. We report here that the transcriptional factor NF-Y is recruited to the duplicated CCAAT box in vivo. A mutation of the duplicated CCAAT box that severely disrupts the NF-Y binding also reduces the accessibility level of the gamma gene promoter, affects the assembly of basal transcriptional machinery, and increases the recruitment of GATA-1 to the locus control region (LCR) and the proximal promoter and the recruitment of transcription cofactor CBP/p300 to the LCR. These findings suggest that recruitment of NF-Y to the duplicated CCAAT box plays a role in the chromatin opening of the gamma gene promoter as well as in the communication between the gamma gene promoter and the LCR.
重复的CCAAT框是γ基因表达所必需的。我们在此报告，转录因子NF-Y在体内被招募至重复的CCAAT框。严重破坏NF-Y结合的重复CCAAT框突变也会降低γ基因启动子的可及性水平，影响基础转录机制的组装，并增加GATA-1至基因座控制区（LCR）和近端启动子的募集以及转录辅因子CBP/p300至LCR的募集。这些发现表明，NF-Y被招募至重复的CCAAT框在γ基因启动子的染色质开放以及γ基因启动子与LCR之间的通讯中发挥作用。
