Yongping G, Jianyun G, Jieshou L
Laboratory of Transplantation Immunology, Nanjing University School of Medicine, Nanjing, People's Republic of China.
Microsurgery. 2001;21(2):55-7. doi: 10.1002/micr.1009.
The objective of this work was to establish a stable and simple simultaneous pancreaticoduodenal-kidney transplantation model in rats. The methods involved harvesting a pancreaticoduodenal-kidney (left) (PDK) and 1-cm inferior vena cava (IVC) with a 0.5-cm left and right iliac communis vein from donors and to "cuff" anastomose between portal vein and right iliac communis vein, left kidney vein, and left iliac communis vein, converging donor portal vein and left kidney vein into IVC together. Next, we performed an anastomosis of the donor arterial segment and recipient abdominal aorta and a "cuff" anastomosis between donor IVC and recipient left kidney vein. Of 67 transplanted rats in which diabetes was induced, 57 survived >7 days, 55 survived 1 month, 54 rats have survived >4 months. In 51 rats, nonfasting plasma glucose levels were euglycemic. We performed three "cuff" anastomoses to simplify the surgical procedure and to shorten the ischemia time of the graft; the recipient vein system has an integrated endovenous membrane to avoid venous thrombi in venous anastomosis sites.
这项工作的目的是在大鼠中建立一种稳定且简单的胰十二指肠-肾脏联合移植模型。方法包括从供体获取胰十二指肠-肾脏（左）（PDK）及一段1厘米长的下腔静脉（IVC），连同0.5厘米长的左右髂总静脉，在门静脉与右髂总静脉、左肾静脉及左髂总静脉之间进行“套袖式”吻合，使供体门静脉和左肾静脉共同汇入IVC。接下来，进行供体动脉段与受体腹主动脉的吻合以及供体IVC与受体左肾静脉之间的“套袖式”吻合。在67只诱导糖尿病的移植大鼠中，57只存活超过7天，55只存活1个月，54只大鼠存活超过4个月。在51只大鼠中，非空腹血浆葡萄糖水平正常。我们进行了三次“套袖式”吻合以简化手术过程并缩短移植物的缺血时间；受体静脉系统具有完整的静脉内膜，以避免静脉吻合部位出现静脉血栓。
