Paczesny S, Beranger S, Salzmann J L, Klatzmann D, Colombo B M
Laboratoire d'Histologie et de Th rapie Génique, Université de Médecine Paris-XIII, Bobigny, France.
Cancer Res. 2001 Mar 15;61(6):2386-9.
Dendritic cells (DCs) are professional antigen-presenting cells (APCs) that need to be activated before they can function to initiate primary and secondary immune responses in vivo. DCs are also specialized to maintain peripheral tolerance to self after uptake of apoptotic material, likely corresponding to both apoptotic bodies and whole apoptotic cells. Here, we report that murine bone marrow-derived DCs can be activated in vitro by exogenous signals received from apoptotic leukemia cells expressing on the cell surface a model tumor-associated antigen. Injected in vivo, these exogenously activated DCs can function as adjuvants to protect mice against leukemia by stimulating an antigen-specific cellular-mediated cytotoxic immune response. To our knowledge, this is the first report indicating that DCs loaded with apoptotic leukemia cells protect mice against leukemia development.
树突状细胞（DCs）是专职抗原呈递细胞（APCs），在体内发挥启动初次和二次免疫反应的功能之前需要被激活。DCs在摄取凋亡物质后也具有维持对外周自身抗原耐受性的特殊功能，这些凋亡物质可能既包括凋亡小体也包括完整的凋亡细胞。在此，我们报道，表达模型肿瘤相关抗原的凋亡白血病细胞所发出的外源性信号可在体外激活小鼠骨髓来源的DCs。将这些外源性激活的DCs注射到体内，它们可以作为佐剂，通过刺激抗原特异性细胞介导的细胞毒性免疫反应来保护小鼠抵抗白血病。据我们所知，这是首次报道表明负载凋亡白血病细胞的DCs可保护小鼠抵抗白血病的发生。
