Kado S, Uchida K, Funabashi H, Iwata S, Nagata Y, Ando M, Onoue M, Matsuoka Y, Ohwaki M, Morotomi M
Yakult Central Institute for Microbiological Research, Tokyo, Japan.
Cancer Res. 2001 Mar 15;61(6):2395-8.
This study was conducted to confirm the hypothesis that intestinal microflora are required for the development of adenocarcinoma in the colon of the TCRbeta and p53 double-knockout (TCRbeta-/- p53-/-) mouse. Germ-free TCRbeta-/- p53-/- mice were produced. At 7 weeks of age, the animals were divided into two groups (n = 10/group), and one of these groups was conventionalized. Animals of both groups were subjected to histopathological examination for adenocarcinoma of the colon at 4 months of age. There was no development of adenocarcinoma of the colon among the germ-free mice, whereas in the conventionalized group, adenocarcinomas of the ileocecum and cecum were detected in 70% of animals. These results indicate the usefulness of the TCRbeta-/- p53-/- mouse as a colon cancer animal model that develops spontaneous adenocarcinoma of the colon early in life, and suggest that intestinal microflora play a major role in the development of adenocarcinoma of the colon in this animal model.
本研究旨在证实肠道微生物群是TCRβ和p53双敲除（TCRβ-/- p53-/-）小鼠结肠腺癌发生所必需的这一假说。培育出了无菌的TCRβ-/- p53-/-小鼠。7周龄时，将动物分为两组（每组n = 10），其中一组进行常规饲养。两组动物在4月龄时均接受了结肠腺癌的组织病理学检查。无菌小鼠中未发生结肠腺癌，而在常规饲养组中，70%的动物检测到回盲部和盲肠腺癌。这些结果表明TCRβ-/- p53-/-小鼠作为一种在生命早期自发发生结肠腺癌的结肠癌动物模型是有用的，并提示肠道微生物群在该动物模型的结肠腺癌发生中起主要作用。
