Kemp D H, Koudstaal D, Roberts J A, Kerr J D
Parasitology. 1975 Apr;70(2):243-54. doi: 10.1017/s0031182000049702.
Larvae of the cattle tick Boophilus microplus will attach to thin (0-3--0-5 mm) slices of cattle skin and engorge on a partially defined medium at 35 degrees C. Forty-seven to 83 per cent of the larvae had engorged by 8 days, and 51--71 per cent of these moulted to nymphs. Tissue culture medium alone allowed little growth unless supplemented with dialysed, freeze dried bovine serum (7 per cent, w/v). This medium could be further difined by substituting purified bovine serum albumin (Cohn fraction V) for the dialysed bovine serum. In one experiment, nymphs of Haemaphysalis longicornis engorged and later moulted to adults. Skin slices were used fresh or after freeze-drying and storing at --25 degrees C. The possible uses of the culture method are discussed.
微小牛蜱幼虫会附着在薄（0.3 - 0.5毫米）的牛皮切片上，并在35摄氏度下于部分确定的培养基中饱食。到第8天时，47%至83%的幼虫已饱食，其中51% - 71%蜕皮为若虫。单独的组织培养基几乎没有生长，除非补充经透析、冻干的牛血清（7%，w/v）。用纯化的牛血清白蛋白（科恩V组分）替代经透析的牛血清可进一步确定这种培养基。在一项实验中，长角血蜱若虫饱食后蜕皮为成虫。皮肤切片可新鲜使用，也可冻干后在 - 25摄氏度下储存。文中讨论了该培养方法的可能用途。
