Alpan O, Rudomen G, Matzinger P
Ghost Lab, Section on T-Cell Tolerance and Memory, Laboratory of Cellular and Molecular Immunology, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
J Immunol. 2001 Apr 15;166(8):4843-52. doi: 10.4049/jimmunol.166.8.4843.
Although induction of T cell responses to fed Ag (oral tolerance) is thought to happen within the organized lymphoid tissue of the gut, we found that mice lacking Peyer's patches, B cells, and the specialized Ag-handling M cells had no defect in the induction of T cell responses to fed Ag, whether assayed in vitro by T cell proliferation or cytokine production, or in vivo by delayed-type hypersensitivity or bystander suppression against mycobacterial Ags in CFA. Feeding of Ag had a major influence on dendritic cells from fed wild-type or muMT mice, such that these APCs were able to elicit a different class of response from naive T cells in vitro. These results suggest that systemic immune responses to soluble oral Ags do not require an organized gut-associated lymphoid tissue but are most likely induced by gut-conditioned dendritic cells that function both to initiate the gut-oriented response and to impart the characteristic features that discriminate it from responses induced parenterally.
尽管人们认为对经口给予抗原（口服耐受）的T细胞应答诱导发生在肠道的有组织淋巴组织内，但我们发现，缺乏派尔集合淋巴结、B细胞以及专门处理抗原的M细胞的小鼠，在对经口给予抗原的T细胞应答诱导方面没有缺陷，无论是通过T细胞增殖或细胞因子产生在体外进行检测，还是通过迟发型超敏反应或对弗氏完全佐剂中分枝杆菌抗原的旁观者抑制在体内进行检测。给予抗原对来自经口给予抗原的野生型或muMT小鼠的树突状细胞有重大影响，以至于这些抗原呈递细胞能够在体外引发来自幼稚T细胞的不同类型应答。这些结果表明，对可溶性口服抗原的全身免疫应答不需要有组织的肠道相关淋巴组织，而很可能是由肠道条件化的树突状细胞诱导的，这些树突状细胞既能启动肠道定向应答，又能赋予其与经肠外诱导的应答相区别的特征。
