Holman S, Collado P
Department of Anatomy, University of Cambridge, Downing Site, Cambridge CB2 3DY, United Kingdom.
J Neurobiol. 2001 May;47(2):150-8. doi: 10.1002/neu.1022.
Sexual differentiation and lateralization of neurone number in a discrete forebrain nucleus (SDApc) related to masculine vocal emission, occur contemporaneously in postnatal (P0-P15) gerbils. Stereological estimates of cell proliferation and death during SDApc organization were made by BrdU labelling and pyknosis, respectively. Results confirmed that rates of apoptosis were greater in females and lateralized in males. Immunoreactive BrdU cells, located in the SDApc at P0-P6, with low levels at P15, were not numerically different between the sexes. Only at one age, P0, in males, was a left-right difference seen in BrdU-immunoreactive cell numbers. Microglia, identified by isolectin immunostaining, were numerically similar to BrdU cells. We suggest that apoptosis, rather than neurogenesis, differentiates and lateralizes SDApc organization, and proliferating cells are microglia, phagocytosing debris.
与雄性发声相关的离散前脑核（SDApc）中神经元数量的性别分化和侧化在出生后（P0 - P15）的沙鼠中同时发生。分别通过BrdU标记和固缩对SDApc组织过程中的细胞增殖和死亡进行体视学估计。结果证实，雌性的细胞凋亡率更高，而雄性的细胞凋亡率呈侧化。位于P0 - P6的SDApc中的免疫反应性BrdU细胞，在P15时水平较低，两性之间在数量上没有差异。仅在雄性的一个年龄阶段P0时，观察到BrdU免疫反应性细胞数量存在左右差异。通过异凝集素免疫染色鉴定的小胶质细胞在数量上与BrdU细胞相似。我们认为，细胞凋亡而非神经发生使SDApc组织发生分化和侧化，并且增殖细胞是小胶质细胞，负责吞噬碎片。
