来自昆虫28S核糖体RNA的多核苷酸片段。
Polynucleotide fragments from the 28S ribosomal RNA of insects.
作者信息
Ishikawa H
出版信息
Nucleic Acids Res. 1975 Jan;2(1):87-100. doi: 10.1093/nar/2.1.87.
If RNA is extracted from the ribosomes which had been isolated from frozen-thawed tissue of Galleria mellonella, the 28 S RNA, when heated or treated with urea, dissociates into seven different species of polynucleotide fragments. They were designated as R1, R2, R3, R4, R5, R6, and R7, whose molecular weights were estimated to be 1.15x10-6, 0.75x10-6, 0.55x10-6, 0.40x10-6, 0.30x10-6, 0.25x10-6, 0.20x10-6 daltons, respectively. It is likely that R1 and R5 arise from a single nick in original 38 S rRNA. Experiments with isolated R1 suggest that it is made up of a hydrogen-bonded complex of R2 and R4. R5 is a complex of R6 and an unidentified species, X. It is suggested that these fragments result from nicks which are introduced, secondarily, in the phosphodiester bonds by an endogenous endonuclease(s). Since the secondary nicks are limited in number and located in specific points of the molecule, it appears that the reaction is quite specific. It was also shown that the 28 S aphid RNA, which apparently lacks the primary nick, is susceptible to nicking.
如果从经冻融处理的大蜡螟组织中分离得到的核糖体提取RNA，28S RNA在加热或用尿素处理时，会解离成七种不同的多核苷酸片段。它们被命名为R1、R2、R3、R4、R5、R6和R7，其分子量估计分别为1.15×10⁻⁶、0.75×10⁻⁶、0.55×10⁻⁶、0.40×10⁻⁶、0.30×10⁻⁶、0.25×10⁻⁶、0.20×10⁻⁶道尔顿。R1和R5可能源自原始38S rRNA上的一个切口。对分离出的R1进行的实验表明，它由R2和R4通过氢键结合形成的复合物组成。R5是R6与一个未鉴定的组分X形成的复合物。有人提出，这些片段是由一种内源性核酸内切酶在磷酸二酯键中继发引入的切口产生的。由于继发切口数量有限且位于分子的特定位置，因此该反应似乎具有相当的特异性。还表明，明显缺乏初级切口的28S蚜虫RNA也易受切口作用。
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