Hofert H F, Laugen R H
Proc Soc Exp Biol Med. 1975 Jan;148(1):187-9. doi: 10.3181/00379727-148-38502.
In short term cultures of rat thymic lymphocytes, theophylline caused an early inhibition of incorporation of 3-H deoxycytidine into DNA without influencing the incorporation of 3-H-thymidine. Over a 4-hr incubation period, the inhibitory effect of theophylline on 3-H-deoxycytidine uptake into DNA remained at approximately 40%, while the suppression of 3-H-thymidine uptake increased to 20%. The results indicate that since there is a difference in the effect of the ophylline on the incorporation of 3-H-deoxycytidine and 3-H-thymidine into the DNA of thymus lymphocytes, inhibition of uptake of these deoxynucleosides by theophylline does not necessarily reflect inhibition of DNA synthesis or cell turnover.
在大鼠胸腺淋巴细胞的短期培养中，茶碱会早期抑制3 - H脱氧胞苷掺入DNA，而不影响3 - H - 胸腺嘧啶核苷的掺入。在4小时的孵育期内，茶碱对3 - H - 脱氧胞苷摄取到DNA中的抑制作用保持在约40%，而对3 - H - 胸腺嘧啶核苷摄取的抑制作用增加到20%。结果表明，由于茶碱对3 - H - 脱氧胞苷和3 - H - 胸腺嘧啶核苷掺入胸腺淋巴细胞DNA的作用存在差异，因此茶碱对这些脱氧核苷摄取的抑制不一定反映DNA合成或细胞更新的抑制。
