Bamberger A M, Sudahl S, Wagener C, Löning T
Institute of Pathology, University Hospital Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.
Int J Gynecol Pathol. 2001 Apr;20(2):160-5. doi: 10.1097/00004347-200104000-00008.
CEACAM1 (CD66a, BGP, C-CAM) is an adhesion molecule of the carcinoembryonic antigen (CEA) family which has been shown to be normally expressed at the apical pole of epithelial cells and to show a dysregulated expression pattern in tumors derived from the latter. The purpose of the present study was to investigate the expression pattern of CEACAM1 in gestational trophoblastic lesions and to compare this expression with the one observed in the normal trophoblast. For this purpose, we performed immunohistochemistry using the 4D1/C2 monoclonal antibody which specifically recognizes CEACAM1 and does not interact with other members of the CEA family. Immunohistochemistry was performed on a total of 20 cases of gestational trophoblastic lesions including complete hydatidiform moles, one placental site trophoblastic nodule (PSN), one placental site trophoblastic tumor (PSTT), and three choriocarcinomas. Immunostaining for cytokeratin, hPL, hCG, and Ki-67 was also performed. Normal placental samples served as a control. CEACAM1 was absent from villous cyto- and syncytiotrophoblast in both normal placenta and hydatidiform molar samples. It was present in the benign extravillous trophoblast, with stronger expression in the proximal extravillous trophoblast of anchoring villi, but was also observed in interstitial and endovascular intermediate trophoblast and chorionic intermediate-like trophoblast. Partial expression was observed in the trophoblast proliferating from the surface of molar villi. In choriocarcinomas, areas of weak expression could be observed along with large areas without CEACAM1 expression. In the PSN and especially in the PSTT, CEACAM1 expression was stronger and more diffuse. The specific localization to extravillous trophoblast and its expression pattern in gestational trophoblastic lesions indicate that CEACAM1 can potentially be a helpful additional diagnostic marker in the differential diagnosis of such lesions.
癌胚抗原相关细胞黏附分子1（CEACAM1，CD66a、BGP、C-CAM）是癌胚抗原（CEA）家族的一种黏附分子，已证实其在正常上皮细胞的顶端极正常表达，而在源自上皮细胞的肿瘤中呈现表达模式失调。本研究的目的是调查CEACAM1在妊娠滋养细胞病变中的表达模式，并将该表达与正常滋养细胞中的表达进行比较。为此，我们使用特异性识别CEACAM1且不与CEA家族其他成员相互作用的4D1/C2单克隆抗体进行免疫组织化学检测。对总共20例妊娠滋养细胞病变进行了免疫组织化学检测，包括完全性葡萄胎、1例胎盘部位滋养细胞结节（PSN）、1例胎盘部位滋养细胞肿瘤（PSTT）和3例绒毛膜癌。还进行了细胞角蛋白、人胎盘催乳素（hPL）、人绒毛膜促性腺激素（hCG）和Ki-67的免疫染色。正常胎盘样本作为对照。在正常胎盘和葡萄胎样本的绒毛细胞滋养层和合体滋养层中均未检测到CEACAM1。它存在于良性绒毛外滋养细胞中，在固定绒毛的近端绒毛外滋养细胞中表达更强，但在间质和血管内中间型滋养细胞以及绒毛膜中间型样滋养细胞中也可观察到。在从葡萄胎绒毛表面增殖的滋养细胞中观察到部分表达。在绒毛膜癌中，可观察到弱表达区域以及大片无CEACAM1表达的区域。在PSN中，尤其是在PSTT中，CEACAM1表达更强且更弥漫。CEACAM1在绒毛外滋养细胞中的特异性定位及其在妊娠滋养细胞病变中的表达模式表明，CEACAM1有可能成为此类病变鉴别诊断中一个有用的辅助诊断标志物。
