筛选和寻找新型DD-肽酶64-575抑制剂。
Screening and search for novel inhibitors of DD-peptidase 64-575.
作者信息
Rozbicka B, Kurzatkowski W
机构信息
Department of Actinomycetes and Fungi Imperfecti, National Institute of Hygiene (NIH), 24 Chocimska Str., 00-791 Warsaw, Poland.
出版信息
Acta Pol Pharm. 2000 Nov;57 Suppl:8-10.
The aim of the work was search for inhibitors of PBPs (penicillin binding proteins, DD-carboxypeptidase/transpeptidase, DD-peptidase). Between 141 tested Streptomyces strains, 25% showed activity of inhibitors production. The inhibitors were produced by selected Streptomyces strains (NIH Culture Collection). The culture supernatants of Streptomyces rimosus B PZH (inhibitor B) and Streptomyces rimosus NRRL 2234 (inhibitor 2234) exhibited the highest inhibition activity. Both inhibitors were purified by the use of: anion exchange chromatography and reversed phase chromatography (HPLC). Inhibitor B is a gamma-lactam compound and inhibitor 2234 is a beta-lactam.
这项工作的目的是寻找青霉素结合蛋白（PBPs，DD - 羧肽酶/转肽酶、DD - 肽酶）的抑制剂。在141株受试链霉菌菌株中，25%表现出产生抑制剂的活性。这些抑制剂由选定的链霉菌菌株（美国国立卫生研究院菌种保藏中心）产生。龟裂链霉菌B PZH（抑制剂B）和龟裂链霉菌NRRL 2234（抑制剂2234）的培养上清液表现出最高的抑制活性。两种抑制剂均通过以下方法纯化：阴离子交换色谱法和反相色谱法（高效液相色谱法）。抑制剂B是一种γ - 内酰胺化合物，抑制剂2234是一种β - 内酰胺。
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