Pole R J, Qi B Q, Beasley S W
Department of Paediatric Surgery, Christchurch Hospital, New Zealand.
Pediatr Surg Int. 2001;17(1):25-8. doi: 10.1007/s003830000448.
Many infants with oesophageal atresia and tracheo-oesophageal fistula (OA/TOF) have associated tracheomalacia (TM), which is one of the reasons for respiratory complications after surgical correction of the atresia. OA/TOF was induced in the offspring of pregnant rats by intraperitoneal injection of adriamycin. Fetuses were harvested by caesarean section. The trachea, oesophagus, lungs, and stomach were removed en bloc and stained for cartilage using Alcian blue. The tracheas were examined, photographed, and relevant parameters pertaining to the tracheal cartilage were measured. Exposure to adriamycin resulted in a range of anatomical defects including OA/TOF (47%) and tracheal agenesis (TA) (41%). Adriamycin-treated fetuses were smaller (P < 0.01), yet had longer tracheas (P < 0.001) than control fetuses. The OA/TOF fetuses had more tracheal cartilage rings than controls (P < 0.01), whereas TA fetuses had fewer (P < 0.001). Both OA/TOF and TA fetuses had more malformed tracheal cartilage rings than controls (P < 0.001 and P < 0.05, respectively). Cartilage in the proximal part of the trachea was most frequently and severely affected (P < 0.05). These observations clarify the structural abnormalities of tracheal cartilage that occur in rat fetuses with OA/TOF or TA induced by adriamycin, and may explain the functional disturbances of TM seen in OA/TOF.
许多患有食管闭锁和气管食管瘘（OA/TOF）的婴儿伴有气管软化（TM），这是食管闭锁手术矫正后发生呼吸并发症的原因之一。通过腹腔注射阿霉素在怀孕大鼠的后代中诱导出OA/TOF。通过剖腹产取出胎儿。将气管、食管、肺和胃整块取出，并用阿尔辛蓝对软骨进行染色。检查气管、拍照并测量与气管软骨相关的参数。暴露于阿霉素会导致一系列解剖学缺陷，包括OA/TOF（47%）和气管发育不全（TA）（41%）。经阿霉素处理的胎儿比对照胎儿更小（P<0.01），但气管更长（P<0.001）。OA/TOF胎儿的气管软骨环比对照组更多（P<0.01），而TA胎儿的气管软骨环较少（P<0.001）。OA/TOF和TA胎儿的气管软骨环畸形均比对照组更多（分别为P<0.001和P<0.05）。气管近端的软骨最常且最严重地受到影响（P<0.05）。这些观察结果阐明了阿霉素诱导的患有OA/TOF或TA的大鼠胎儿气管软骨的结构异常，并可能解释了OA/TOF中所见的TM功能障碍。
