Riss T L
Division of Cellular Regulation, Promega Corporation, Madison, Wisconsin 53711, USA
Urology. 2001 Apr;57(4 Suppl 1):141-2. doi: 10.1016/s0090-4295(00)00960-2.
Monitoring apoptosis in histologic samples is becoming increasingly important as more effort is applied to the study of therapeutic modulators of cell death in tumors. The common methods used to monitor DNA fragmentation and cell morphology as markers of apoptosis have their own set of advantages and disadvantages. Growing knowledge of the signaling events that occur during cell death has established the caspase enzymes as the central executioner proteases of apoptosis. The results of caspase activity generate neo-epitopes that occur only during apoptosis. Directly monitoring caspase-mediated events as markers of apoptosis offers advantages over existing assay methods. Recently, several new marker antibodies have been developed that detect active caspase enzymes or the products they produce. The possibility of using these new marker antibodies for monitoring prostate chemoprevention trials is presented in this review.
随着越来越多的精力投入到肿瘤细胞死亡治疗调节剂的研究中，监测组织学样本中的细胞凋亡变得越来越重要。用于监测DNA片段化和细胞形态作为细胞凋亡标志物的常用方法都有其自身的优缺点。对细胞死亡过程中发生的信号事件的认识不断增加，已将半胱天冬酶确立为细胞凋亡的核心执行蛋白酶。半胱天冬酶活性的结果产生仅在细胞凋亡期间出现的新表位。直接监测半胱天冬酶介导的事件作为细胞凋亡的标志物比现有检测方法具有优势。最近，已经开发出几种新的标志物抗体，可检测活性半胱天冬酶或它们产生的产物。本综述介绍了使用这些新的标志物抗体监测前列腺化学预防试验的可能性。
