Zhao Y, Brown T L, Kohler H, Müller S
Immpheron Inc., Lexington, KY 40503, USA.
Apoptosis. 2003 Dec;8(6):631-7. doi: 10.1023/A:1026139627930.
Caspase 3 is critically involved in the pathway of apoptosis. We have conjugated a MTS-transport-peptide to monoclonal and polyclonal anti-caspase-3 antibodies to suppress Actinomycin D-induced apoptosis in human lymphoma T cells. The advantage of using trans-membrane antibodies compared to conventional apoptosis inhibitors is their specific target recognition in the living cell and their lower toxicity compared to conventional apoptosis inhibitors. We could show that a MTS-transport-peptide modified monoclonal anti-caspase-3 antibody reduces Actinomycin D induced apoptosis, as shown by DNA ladder electrophoresis and cell death ELISA. These results indicate that antibodies have a therapeutic potential to inhibit apoptosis in a variety of diseases.
半胱天冬酶3在细胞凋亡途径中起关键作用。我们已将一种MTS转运肽与单克隆和多克隆抗半胱天冬酶-3抗体偶联，以抑制放线菌素D诱导的人淋巴瘤T细胞凋亡。与传统的凋亡抑制剂相比，使用跨膜抗体的优势在于其在活细胞中的特异性靶点识别以及与传统凋亡抑制剂相比更低的毒性。我们能够证明，如DNA梯状电泳和细胞死亡ELISA所示，一种MTS转运肽修饰的单克隆抗半胱天冬酶-3抗体可减少放线菌素D诱导的凋亡。这些结果表明，抗体在抑制多种疾病中的细胞凋亡方面具有治疗潜力。
