SDE3编码一种拟南芥转录后基因沉默所需的RNA解旋酶。
SDE3 encodes an RNA helicase required for post-transcriptional gene silencing in Arabidopsis.
作者信息
Dalmay T, Horsefield R, Braunstein T H, Baulcombe D C
机构信息
The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
出版信息
EMBO J. 2001 Apr 17;20(8):2069-78. doi: 10.1093/emboj/20.8.2069.
Abstract
Post-transcriptional gene silencing (PTGS) provides protection in plants against virus infection and can suppress expression of transgenes. Arabidopsis plants carrying mutations at the SDE3 locus are defective in PTGS mediated by a green fluorescent protein transgene. However, PTGS mediated by tobacco rattle virus (TRV) was not affected by sde3. From these results we conclude that SDE3, like the previously described RNA polymerase encoded by SDE1, acts at a stage in the mechanism that is circumvented when PTGS is mediated by TRV. The product of SDE3 is similar to RNA helicase-like proteins including GB110 in mouse and other proteins in Drosophila and humans. These proteins are similar to, but clearly distinct from Upf1p and SMG-2, which are required for nonsense-mediated mRNA decay in yeast and Caenorhabditis elegans and, in the case of SMG-2, for PTGS.
摘要
转录后基因沉默（PTGS）为植物提供了抵御病毒感染的保护作用，并且能够抑制转基因的表达。在SDE3基因座携带突变的拟南芥植株在由绿色荧光蛋白转基因介导的PTGS中存在缺陷。然而，由烟草脆裂病毒（TRV）介导的PTGS不受sde3的影响。从这些结果我们得出结论，SDE3与先前描述的由SDE1编码的RNA聚合酶一样，在由TRV介导PTGS时所规避的机制阶段发挥作用。SDE3的产物与RNA解旋酶样蛋白相似，包括小鼠中的GB110以及果蝇和人类中的其他蛋白。这些蛋白与Upf1p和SMG-2相似，但明显不同，Upf1p和SMG-2是酵母和秀丽隐杆线虫中无义介导的mRNA降解所必需的，就SMG-2而言，是PTGS所必需的。
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An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus.拟南芥中的一个依赖RNA的RNA聚合酶基因对于由转基因介导而非病毒介导的转录后基因沉默是必需的。
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