Activation-dependent changes in receptor distribution and dendritic morphology in hippocampal neurons expressing P2X2-green fluorescent protein receptors.
作者信息
Khakh B S, Smith W B, Chiu C S, Ju D, Davidson N, Lester H A
机构信息
Division of Biology, 156-29, California Institute of Technology, Pasadena, CA 91125, USA.
出版信息
Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5288-93. doi: 10.1073/pnas.081089198. Epub 2001 Apr 10.
Abstract
ATP-gated P2X(2) receptors are widely expressed in neurons, but the cellular effects of receptor activation are unclear. We engineered functional green fluorescent protein (GFP)-tagged P2X(2) receptors and expressed them in embryonic hippocampal neurons, and report an approach to determining functional and total receptor pool sizes in living cells. ATP application to dendrites caused receptor redistribution and the formation of varicose hot spots of higher P2X(2)-GFP receptor density. Redistribution in dendrites was accompanied by an activation-dependent enhancement of the ATP-evoked current. Substate-specific mutant T18A P2X(2)-GFP receptors showed no redistribution or activation-dependent enhancement of the ATP-evoked current. Thus fluorescent P2X(2)-GFP receptors function normally, can be quantified, and reveal the dynamics of P2X(2) receptor distribution on the seconds time scale.
摘要
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本文引用的文献
1
Molecular physiology of P2X receptors and ATP signalling at synapses.
Nat Rev Neurosci. 2001 Mar;2(3):165-74. doi: 10.1038/35058521.
2
International union of pharmacology. XXIV. Current status of the nomenclature and properties of P2X receptors and their subunits.
Pharmacol Rev. 2001 Mar;53(1):107-18.
3
Single-molecule measurements calibrate green fluorescent protein surface densities on transparent beads for use with 'knock-in' animals and other expression systems.
J Neurosci Methods. 2001 Jan 30;105(1):55-63. doi: 10.1016/s0165-0270(00)00354-x.
4
Distinct Localization of P2X receptors at excitatory postsynaptic specializations.
J Neurosci. 2001 Jan 15;21(2):641-53. doi: 10.1523/JNEUROSCI.21-02-00641.2001.
5
Expression of P2X(7) purinoceptors on human lymphocytes and monocytes: evidence for nonfunctional P2X(7) receptors.
Am J Physiol Cell Physiol. 2000 Oct;279(4):C1189-97. doi: 10.1152/ajpcell.2000.279.4.C1189.
6
Single channel properties of P2X ATP receptors in outside-out patches from rat hippocampal granule cells.
J Physiol. 2000 Sep 15;527 Pt 3(Pt 3):529-47. doi: 10.1111/j.1469-7793.2000.00529.x.
7
Identification of amino acid residues contributing to the ATP-binding site of a purinergic P2X receptor.
J Biol Chem. 2000 Nov 3;275(44):34190-6. doi: 10.1074/jbc.M005481200.
8
State-dependent cross-inhibition between transmitter-gated cation channels.
Nature. 2000 Jul 27;406(6794):405-10. doi: 10.1038/35019066.
9
Functional properties of heteromeric P2X(1/5) receptors expressed in HEK cells and excitatory junction potentials in guinea-pig submucosal arterioles.
J Auton Nerv Syst. 2000 Jul 3;81(1-3):249-63. doi: 10.1016/s0165-1838(00)00123-5.
10
The distribution of P2X receptor clusters on individual neurons in sympathetic ganglia and their redistribution on agonist activation.
J Biol Chem. 2000 Sep 15;275(37):29107-12. doi: 10.1074/jbc.M910277199.
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