Liu P C, Chen Y C, Lee K K
Department of Aquaculture, National Taiwan Ocean University, Keelung, Republic of China.
Microbios. 2001;104(408):71-7.
Outbreaks of mass mortality among cultured small abalone Haliotis diversicolor supertexta with abscess/ulcers in the mantle occurred in 1998 at Kao-Hsiung, Taiwan. A swarming bacterium, strain H-11 was isolated from the haemolymph of the moribund small abalone using tryptic soy agar supplemented with 3% NaCl and/or thiosulphate citrate bile salt sucrose agar. This strain was characterized and identified as Vibrio alginolyticus on the basis of various biochemical tests. The H-11 strain and its extracellular products were virulent to small abalones with LD50 values of 3.6 x 10(5) colony forming units and 2.96 microg protein/g body weight, respectively.
1998年，台湾高雄养殖的杂色鲍（Haliotis diversicolor supertexta）出现大规模死亡疫情，患病个体外套膜有脓肿/溃疡。从濒死杂色鲍的血淋巴中，使用添加3%氯化钠的胰蛋白胨大豆琼脂和/或硫代硫酸盐柠檬酸盐胆盐蔗糖琼脂，分离出一种优势菌，即H-11菌株。通过各种生化试验对该菌株进行了表征，并鉴定为溶藻弧菌。H-11菌株及其胞外产物对杂色鲍具有毒性，半数致死剂量分别为3.6×10⁵ 菌落形成单位和2.96微克蛋白质/克体重。
