从患病日本对虾（Penaeus japonicus）中分离出的溶藻弧菌的分离与鉴定

Isolation and characterization of Vibrio alginolyticus isolated from diseased kuruma prawn, Penaeus japonicus.

作者信息

Lee K K, Yu S R, Yang T I, Liu P C, Chen F R

机构信息

Department of Aquaculture, National Taiwan Ocean University, Keelung, ROC.

出版信息

Lett Appl Microbiol. 1996 Feb;22(2):111-4. doi: 10.1111/j.1472-765x.1996.tb01121.x.

DOI:10.1111/j.1472-765x.1996.tb01121.x

PMID:8936369

Abstract

Outbreaks of serious mortality among cultured kuruma prawns (Penaeus japonicus) with white spotted syndrome in the carapace occurred in the summer of 1993 in I-Lan, Taiwan. A swarming bacterium, strain Swy, was isolated from the hepatopancreas of the moribund prawns using tryptic soy agar supplemented with 1% NaCl and/or thiosulphate citrate bile salt sucrose agar. This strain was characterized and identified as Vibrio alginolyticus on the basis of a number of biochemical tests. The Swy strain was virulent to both kuruma prawns (P. japonicus) and tiger prawns (P. monodon) with LD50 values of 4.43 x 10(4) and 1.57 x 10(5) cfu g body weight-1, respectively.

摘要

1993年夏天，台湾宜兰养殖的日本对虾（Penaeus japonicus）出现了严重的死亡疫情，病虾甲壳出现白斑综合征。从濒死对虾的肝胰腺中分离出一种优势菌，菌株Swy，使用添加1%氯化钠的胰蛋白胨大豆琼脂和/或硫代硫酸盐柠檬酸盐胆盐蔗糖琼脂进行分离。通过多项生化试验对该菌株进行了鉴定，确定为溶藻弧菌。Swy菌株对日本对虾（P. japonicus）和斑节对虾（P. monodon）均具有毒性，LD50值分别为4.43 x 10(4)和1.57 x 10(5) cfu g体重-1。