Tohoyama H, Kadota H, Shiraishi E, Inouhe M, Joho M
Department of Biology, Faculty of Science, Ehime University, Bunkyo, Matsuyama, Japan.
Microbios. 2001;104(408):99-104.
Induction for the expression of the metallothionein gene, CUP1, in the yeast Saccharomyces cerevisiae by cobalt was examined using a reporter gene with the promoter of this gene fused to the coding region of lacZ. The expression of the gene was induced by cobalt as well as by copper and silver ions. The activity of beta-galactosidase showed high levels after treatment with 1.0 mM cobalt chloride. It has been reported that the induction for the transcription of CUP1 by copper and silver is mediated by the Ace1 transcription factor. However, the expression of the gene by cobalt occurred in yeast cells lacking the Ace1 factor. These results suggest the presence of a novel cobalt-specific transcription factor for the CUP1 gene.
利用一个报告基因（该基因的启动子与lacZ的编码区融合），研究了钴对酿酒酵母中金属硫蛋白基因CUP1表达的诱导作用。该基因的表达可被钴以及铜离子和银离子诱导。用1.0 mM氯化钴处理后，β-半乳糖苷酶的活性呈现高水平。据报道，铜和银对CUP1转录的诱导是由Ace1转录因子介导的。然而，钴诱导该基因的表达发生在缺乏Ace1因子的酵母细胞中。这些结果表明存在一种新的CUP1基因钴特异性转录因子。
