犬类细胞上CD61（β3整合素亚基）的表达。

Expression of CD61 (beta3 integrin subunit) on canine cells.

作者信息

Arce C, Moreno A, Pérez de la Lastra J M, Garrido J J, Barbancho M, De Andrés D F, Morera L, Llanes D

机构信息

Departamento de Genética, Facultad de Veterinaria, Universidad de Córdoba, Spain.

出版信息

Platelets. 2001 Mar;12(2):69-73. doi: 10.1080/09537100020032828.

DOI:10.1080/09537100020032828

PMID:11297034

Abstract

A monoclonal antibody (JM2E5) specific for the integrin beta3 chain, or CD61 or GPIIIa subunit, has been employed to determine the expression of the canine homologue CD41/CD61 or CD51/CD61 complex on different canine cells in peripheral blood lymphocytes, monocytes, granulocytes, platelets, erythrocytes, lymph-node cells, spleen cells and breast tumour cells). The canine homologue CD41/CD61 or CD51/61 was present on peripheral blood lymphocytes, monocytes, granulocytes, breast tumour cells and spleen cells as well as on platelets and it was absent from erythrocytes and lymph-node cells. An antigen with components of molecular masses of 25/100/120 kDa (under reducing conditions) was immunoprecipitated from canine peripheral lymphocytes and platelets, but not from granulocytes or monocytes. Expression on canine lymphocytes of the canine homologue of the human beta3 integrin chain was unexpected, based on the expression pattern of this molecule in human tissue.

摘要

一种针对整合素β3链或CD61或糖蛋白IIIa亚基的单克隆抗体（JM2E5）已被用于确定犬类同源物CD41/CD61或CD51/CD61复合物在不同犬类细胞上的表达，这些细胞包括外周血淋巴细胞、单核细胞、粒细胞、血小板、红细胞、淋巴结细胞、脾细胞和乳腺肿瘤细胞。犬类同源物CD41/CD61或CD51/61存在于外周血淋巴细胞、单核细胞、粒细胞、乳腺肿瘤细胞和脾细胞以及血小板上，而在红细胞和淋巴结细胞中不存在。在还原条件下，从犬外周淋巴细胞和血小板中免疫沉淀出一种分子量为25/100/120 kDa的抗原成分，但从粒细胞或单核细胞中未沉淀出该抗原。基于该分子在人体组织中的表达模式，人β3整合素链的犬类同源物在犬淋巴细胞上的表达出乎意料。