Ali-Vehmas T, Vikerpuur M, Pyörälä S, Atroshi F
Department of Microbiology, Faculty of Veterinary Medicine, University of Helsinki, Finland.
Microbiol Res. 2001 Mar;155(4):339-44. doi: 10.1016/S0944-5013(01)80013-6.
Beta (beta) and delta (delta)-hemolysin of Staphylococcus aureus strains were cultured in vitro in milk lactoserum (whey) prepared from both healthy and mastitis bovine milk. Production of beta- and delta-hemolysins were detected in 12 out of 50 strains studied. The association between N-acetyl-beta-D-glucosaminidase (NAGase) activity, plasmin activity (PL) and trypsin inhibitory capacity (TIC), known as inflammatory indicators for mastitis, and hemolytic activity were also studied. Mastitic milk decreased directly the lytic effect of both beta-and delta-hemolysins of S. aureus on hemolytical blood agar plates. S. aureus in healthy milk samples produced more beta-hemolysin (3 times) and delta-hemolysin (2 times) when compared to S. aureus supernatants in milk from infected quarters. Furthermore, beta- and delta-hemolysis correlated negatively with TIC and NAGase and PL activities. Addition of reduced glutathione (GSH) or beta-mercaptoethanol into the artificial medium enhanced hemolysins activity.
金黄色葡萄球菌菌株的β（β）和δ（δ）溶血素在由健康奶牛和患乳腺炎奶牛的牛奶制备的乳清乳糖血清中进行体外培养。在所研究的50株菌株中，有12株检测到β和δ溶血素的产生。还研究了作为乳腺炎炎症指标的N - 乙酰 - β - D - 氨基葡萄糖苷酶（NAGase）活性、纤溶酶活性（PL）和胰蛋白酶抑制能力（TIC）与溶血活性之间的关联。患乳腺炎的牛奶直接降低了金黄色葡萄球菌的β和δ溶血素对溶血血琼脂平板的溶解作用。与感染乳腺的牛奶中的金黄色葡萄球菌上清液相比，健康牛奶样本中的金黄色葡萄球菌产生更多的β溶血素（3倍）和δ溶血素（2倍）。此外，β和δ溶血与TIC以及NAGase和PL活性呈负相关。向人工培养基中添加还原型谷胱甘肽（GSH）或β - 巯基乙醇可增强溶血素活性。
