Calaf G, Russo J, Alvarado M E
Center for Radiological Research, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA.
J Submicrosc Cytol Pathol. 2000 Oct;32(4):535-45.
The neoplastic conversion of a normal cell to a malignant one is a multistage process that occurs after a series of molecular alterations. Several chemical and physical agents can alter the morphology of different types of cells. Scanning and transmission electron microscopy have been valuable in evaluating changes that occur in the progression of transformation. MCF-10F, a spontaneously immortalized human breast epithelial cell line (Soule et al., 1990), was treated with benz(alpha)pyrene (BP) (Calaf and Russo, 1993) and then transfected with the c-Ha-ras oncogene (Calaf et al., 1995). The phenotypic changes of breast cancer progression were studied through the use of scanning and transmission electron microscopy. Activated oncogenes have been detected in a variety of malignant tumors and the altered expression of certain genes seems to play a role in the cancer process. Carcinogen-treated and transfected cells showed a progression of changes in the morphology, anchorage independent growth, invasiveness and capability of tumor formation in the SCID mice. This in vitro cancer model can parallel the progression of breast cancer seen through molecular changes that occur and have been observed during the natural development of this disease.
正常细胞向恶性细胞的肿瘤转化是一个多阶段过程，发生在一系列分子改变之后。几种化学和物理因素可改变不同类型细胞的形态。扫描电子显微镜和透射电子显微镜在评估转化过程中发生的变化方面很有价值。MCF-10F是一种自发永生化的人乳腺上皮细胞系（苏尔等人，1990年），用苯并(a)芘（BP）处理（卡拉夫和鲁索，1993年），然后用c-Ha-ras癌基因转染（卡拉夫等人，1995年）。通过扫描电子显微镜和透射电子显微镜研究乳腺癌进展的表型变化。在多种恶性肿瘤中检测到激活的癌基因，某些基因的表达改变似乎在癌症过程中起作用。经致癌物处理和转染的细胞在形态、锚定非依赖性生长、侵袭性以及在SCID小鼠中的肿瘤形成能力方面表现出一系列变化。这种体外癌症模型可以与通过该疾病自然发展过程中发生并观察到的分子变化所看到的乳腺癌进展相平行。
