O'Brien T P, Li Q J, Sauerburger F, Reviglio V E, Rana T, Ashraf M F
Ocular Microbiology and Immunology Laboratory, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, Woods Bldg./Rm. 259, Baltimore, MD 21287-9121, USA.
Ophthalmology. 2001 Apr;108(4):656-9. doi: 10.1016/s0161-6420(00)00590-x.
To investigate the role of matrix metalloproteinases (MMPs) in the pathogenesis of ulcerative keratolysis associated with topical use of generic diclofenac preoperatively and postoperatively. To characterize the inflammatory response of the cornea in this case of ulcerative keratolysis.
Case report with clinicopathologic correlation.
Corneal culture for microbial growth. Clinical and histopathologic examinations including routine histolopathologic, immunofluorescent, and immunohistochemical studies.
Microscopic examination of the corneal button disclosed fibrinous material with neutrophils and mononuclear inflammatory cells. The corneal epithelial basement membrane was irregularly thickened and patchy. Immunohistochemical staining detected weak staining of MMP-1 and a strong presence of MMP-8 in the epithelium. MMP-8 and 9 were also present in areas of leukocytic infiltration. MMP-2 appeared in a few stromal cells. Macrophages and leukocytes were the predominant infiltrating cells.
A nonspecific inflammatory response occurred in this case of ulcerative keratolysis. Corneal epithelial cells are capable of secreting MMP-1 and 8 and may participate in the stromal degradation and repair process of the ulcerative keratolysis associated with topical nonsteroidol antiinflammatory use.
研究基质金属蛋白酶（MMPs）在术前及术后局部使用非专利型双氯芬酸相关的溃疡性角膜溶解发病机制中的作用。对该溃疡性角膜溶解病例中的角膜炎症反应进行特征描述。
伴有临床病理相关性的病例报告。
角膜微生物生长培养。临床及组织病理学检查，包括常规组织病理学、免疫荧光及免疫组织化学研究。
对角膜植片的显微镜检查发现有含中性粒细胞和单核炎性细胞的纤维蛋白物质。角膜上皮基底膜不规则增厚且呈片状。免疫组织化学染色检测到上皮中MMP-1染色较弱，MMP-8大量存在。MMP-8和9也存在于白细胞浸润区域。MMP-2出现在少数基质细胞中。巨噬细胞和白细胞是主要的浸润细胞。
该溃疡性角膜溶解病例发生了非特异性炎症反应。角膜上皮细胞能够分泌MMP-1和8，并可能参与与局部使用非甾体抗炎药相关的溃疡性角膜溶解的基质降解和修复过程。
