Burleigh S H.
Department of Biology and Biogeochemistry, Risø National Laboratory, DK40000, Roskilde, Denmark
Plant Sci. 2001 Apr;160(5):899-904. doi: 10.1016/s0168-9452(00)00460-x.
The influence of arbuscular mycorrhizal fungi (AMF) on the expression of plant nutrient transporters was studied using a relative, quantitative reverse-transcription polymerase chain-reaction (RQRT-PCR) technique. Reverse-transcribed 18S rRNA was used to standardize the treatments. The technique had high reproducibility and reflected trends in gene expression as observed by Northern blotting. Using this technique, it was demonstrated that both the high-affinity phosphate transporter MtPt2 and a putative nitrate transporter from Medicago truncatula were down-regulated in roots when colonized by some, but not all AMF. Colonization by the AMF Glomus rosea, in particular, failed to strongly down-regulate these plant genes within the root. This technique may be suitable for the study of plant genes in mycorrhizal roots when Northern blotting is not possible due to low gene expression or when limited amounts of tissue are available for study.
利用相对定量逆转录聚合酶链反应（RQRT-PCR）技术，研究了丛枝菌根真菌（AMF）对植物养分转运蛋白表达的影响。使用逆转录的18S rRNA对处理进行标准化。该技术具有高重现性，并能反映出如Northern印迹法所观察到的基因表达趋势。运用此技术证明，当被部分而非全部AMF定殖时，紫花苜蓿的高亲和力磷酸盐转运蛋白MtPt2和一种假定的硝酸盐转运蛋白在根中表达下调。特别是，AMF根内球囊霉的定殖未能强烈下调根内的这些植物基因。当由于基因表达水平低而无法进行Northern印迹法，或者可用于研究的组织量有限时，该技术可能适用于研究菌根根中的植物基因。
