Voitenko L P, Voitenko S V, Skok M V, Purnyn H E, Skok V I
Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, 4, Bogomoletz str., 01024 Kiev, Ukraine.
Neurosci Lett. 2001 Apr 27;303(1):37-40. doi: 10.1016/s0304-3940(01)01705-0.
All cultured neurons of rat superior cervical ganglion (SCG) were stained with nicotinic acetylcholine receptor (nAChR) alpha5- or alpha7-subunit-specific oligoclonal antibodies (Abs) and could additionally bind alpha3-subunit-specific monoclonal antibody (mAb). About 60% of the neurons were stained with alpha4-specific Ab and could not bind alpha3-specific mAb. The acetylcholine-induced membrane currents recorded with the whole-cell patch clamp method and partially blocked with alpha3-specific mAbs, could be additionally blocked with alpha5- and alpha7- specific Abs, and vice versa. The results suggest that: (1) each neuron of rat SCG expresses several nAChR subtypes with different alpha-subunits; (2) the alpha3-, alpha5- and alpha7-subunit-containing nAChRs are probably located far enough from each other thus enabling joint binding to the cell of the corresponding alpha-subunit specific Abs, in contrast to the alpha4-subunit-containing nAChRs which are probably located too close to the alpha3-containing ones to allow their joint binding.
大鼠颈上神经节（SCG）的所有培养神经元均用烟碱型乙酰胆碱受体（nAChR）α5或α7亚基特异性寡克隆抗体（Abs）染色，并且还能与α3亚基特异性单克隆抗体（mAb）结合。约60%的神经元用α4特异性抗体染色，且不能与α3特异性单克隆抗体结合。采用全细胞膜片钳法记录的乙酰胆碱诱导的膜电流，部分被α3特异性单克隆抗体阻断，还可被α5和α7特异性抗体进一步阻断，反之亦然。结果表明：（1）大鼠SCG的每个神经元表达几种具有不同α亚基的nAChR亚型；（2）含α3、α5和α7亚基的nAChRs可能彼此相距足够远，从而能够与相应α亚基特异性抗体联合结合到细胞上，而含α4亚基的nAChRs可能与含α3亚基的nAChRs位置太近，以至于不能联合结合。
