Altairac S, Chaudun E, Courtois Y, Torriglia A
Unité 450 INSERM, Affiliée CNRS, Association Claude Bernard, 29 rue Wilhem, 75016 Paris, France.
Neurosci Lett. 2001 Apr 27;303(1):41-4. doi: 10.1016/s0304-3940(01)01706-2.
During retinal development, the neuronal death is carried out by the mechanism of apoptosis. Among the different endonucleases activated, L-DNase II seems to be responsible for most of DNA degradation in this tissue. L-DNase II derives from LEI (Leukocyte Elastase Inhibitor) by a post-translational modification carried out by elastase in apoptosis induced in vitro. In this study, we investigated whether elastase could be implicated in apoptosis occurring during retinal development. Although elastase and LEI/elastase complex are colocalized in retinal sections, the LEI/elastase complex, detected by Western blot, does not change at all stages of development. However, at pH 4 retinal extracts show an enhanced activation of the L-DNase II. These results suggest that an acid protease, such as a cathepsin, may be implicated in neuronal retinal apoptosis.
在视网膜发育过程中，神经元死亡是通过凋亡机制进行的。在激活的不同核酸内切酶中，L-DNase II似乎负责该组织中大部分的DNA降解。L-DNase II是由LEI（白细胞弹性蛋白酶抑制剂）通过弹性蛋白酶在体外诱导凋亡过程中进行的翻译后修饰产生的。在本研究中，我们调查了弹性蛋白酶是否与视网膜发育过程中发生的凋亡有关。尽管弹性蛋白酶和LEI/弹性蛋白酶复合物在视网膜切片中共定位，但通过蛋白质印迹检测到的LEI/弹性蛋白酶复合物在发育的所有阶段都没有变化。然而，在pH 4时，视网膜提取物显示L-DNase II的激活增强。这些结果表明，一种酸性蛋白酶，如组织蛋白酶，可能与视网膜神经元凋亡有关。
