Jenne L, Arrighi J F, Sauter B, Kern P
Department of Dermatology, University Hospital and Medical Clinic Erlangen-Nuremberg, Germany.
Parasite Immunol. 2001 Apr;23(4):195-201. doi: 10.1046/j.1365-3024.2001.00374.x.
Dendritic cells (DC) are sentinels of immunity. We determined their role in the induction of immunity against alveolar echinococcosis, caused by the larval stage of the cestode Echinococcus multilocularis. Furthermore, we evaluated if unfractionated protein from E. multilocularis (Em-Ag) can be used as loading agent for DC (comparable to unfractionated tumour proteins) in order to generate antiparasitic cytotoxic T lymphocyte (CTL). Interestingly, immature DC did not mature in the presence of 1 microg/ml Em-Ag as analysed by FACS and mixed leucocyte reactions. Yet, their capacity to take up dextran was markedly reduced. Further maturation of immature Em-Ag pulsed DC could be induced by proinflammatory cytokines. These mature DC were slightly better inducers of T cell proliferation when compared with unpulsed mature DC. Importantly, by repetetive stimulation of autologous CD8+ lymphocytes with the Em-Ag pulsed mature DC, we were able to generate specifically proliferating CTL lines. Thus, immunotherapy with ex vivo generated Em-Ag pulsed DC might be of benefit for patients inheriting this incurable disease.
树突状细胞（DC）是免疫的哨兵。我们确定了它们在诱导针对由多房棘球绦虫幼虫阶段引起的肺泡棘球蚴病的免疫中的作用。此外，我们评估了多房棘球绦虫的未分级蛋白质（Em-Ag）是否可以用作DC的负载剂（类似于未分级的肿瘤蛋白质）以产生抗寄生虫细胞毒性T淋巴细胞（CTL）。有趣的是，通过流式细胞术和混合淋巴细胞反应分析，未成熟DC在存在1μg/ml Em-Ag的情况下未成熟。然而，它们摄取葡聚糖的能力明显降低。促炎细胞因子可诱导未成熟的Em-Ag脉冲DC进一步成熟。与未脉冲的成熟DC相比，这些成熟DC对T细胞增殖的诱导作用稍好。重要的是，通过用Em-Ag脉冲的成熟DC重复刺激自体CD8 +淋巴细胞，我们能够产生特异性增殖的CTL系。因此，用体外产生的Em-Ag脉冲DC进行免疫治疗可能对患有这种不治之症的患者有益。
