Vásquez A, Ahrné S, Pettersson B, Molin G
Laboratory of Food Hygiene, Division of Food Technology, Lund University, Sweden.
Lett Appl Microbiol. 2001 Apr;32(4):215-9. doi: 10.1046/j.1472-765x.2001.00901.x.
To develop a tool for rapid and inexpensive identification of the Lactobacillus casei complex.
Lactobacillus casei, Lactobacillus paracasei, Lactobacillus zeae and Lactobacillus rhamnosus were identified by PCR-amplification of the segment between the U1 and U2 regions of 16S rDNA (position 8-357, Escherichia coli numbering) and temporal temperature gradient gel electrophoresis (TTGE). Seven tested Lact. paracasei strains were divided into three TTGE-subgroups.
TTGE successfully distinguished between the closely-related target species. TTGE is also a powerful method for revealing sequence heterogeneities in the 16S rRNA genes.
Due to rapid and easy performance, TTGE of PCR-amplified 16S rDNA fragments will be useful for the identification of extended numbers of isolates.
开发一种用于快速且低成本鉴定干酪乳杆菌复合体的工具。
通过对16S rDNA的U1和U2区域之间的片段（大肠杆菌编号为8 - 357位）进行PCR扩增以及时间温度梯度凝胶电泳（TTGE）来鉴定干酪乳杆菌、副干酪乳杆菌、玉米乳杆菌和鼠李糖乳杆菌。7株受试副干酪乳杆菌菌株被分为3个TTGE亚组。
TTGE成功区分了密切相关的目标物种。TTGE也是揭示16S rRNA基因序列异质性的有力方法。
由于操作快速简便，PCR扩增的16S rDNA片段的TTGE将有助于鉴定更多数量的分离株。
