Feldheim K A, Gruber S H, Ashley M V
Department of Biological Sciences, University of Illinois at Chicago, 845 West Taylor Street, Chicago, IL 60076, USA.
Mol Ecol. 2001 Feb;10(2):295-303. doi: 10.1046/j.1365-294x.2001.01182.x.
DNA microsatellite markers were used to characterize the population genetic structure of the lemon shark, Negaprion brevirostris, in the western Atlantic. This study demonstrates for the first time the usefulness of microsatellites to study population genetic structure and mating systems in the Chondricthyes. Lemon sharks (mostly juveniles) were sampled non-destructively from four locations, Gullivan Bay and Marquesas Key in Florida, Bimini, Bahamas, and Atol das Rocas, Brazil. At least 545 individuals were genotyped at each of four dinucleotide loci. The number of alleles per locus ranged from 19 to 43, and expected heterozygosities ranged from 0.69 to 0.90. Relatively little genetic structure was found in the western Atlantic, with small but significant values for estimators of F(ST) and R(ST) among populations, theta (0.016) and rho (0.026), respectively. No sharp discontinuities were found between the Caribbean sites and Brazil, and most alleles were found at all four sites, indicating that gene flow occurs throughout the western Atlantic with no evidence for distinct stocks.
DNA微卫星标记被用于描述西大西洋柠檬鲨（Negaprion brevirostris）的种群遗传结构。本研究首次证明了微卫星在研究软骨鱼类种群遗传结构和交配系统方面的实用性。柠檬鲨（主要是幼鲨）从四个地点进行了非破坏性采样，分别是佛罗里达州的古利文湾和马奎萨斯礁、巴哈马的比米尼以及巴西的罗卡斯环礁。在四个二核苷酸位点上，每个位点至少对545个个体进行了基因分型。每个位点的等位基因数量在19到43之间，预期杂合度在0.69到0.90之间。在西大西洋发现的遗传结构相对较少，种群间F(ST)和R(ST)的估计值分别为较小但显著的θ（0.016）和ρ（0.026）。在加勒比地区的地点和巴西之间未发现明显的间断，且在所有四个地点都发现了大多数等位基因，这表明整个西大西洋都存在基因流动，没有证据表明存在不同的种群。
