乙酰唑胺对嗜糖小球藻碳浓缩机制的抑制作用。

The inhibition of the carbon concentrating mechanism of the green alga Chlorella saccharophila by acetazolamide.

作者信息

Pollock Steve Vincent, Colman Brian

机构信息

Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA; Department of Biology, York University, 4700 Keele St., Toronto, Ontario, M3J 1P3, Canada.

出版信息

Physiol Plant. 2001 Apr;111(4):527-532. doi: 10.1034/j.1399-3054.2001.1110413.x.

DOI:10.1034/j.1399-3054.2001.1110413.x

PMID:11299018

Abstract

The effects of the carbonic anhydrase (CA) inhibitors acetazolamide (AZ) and dextran-bound sulfonamide (DBS) on HCO3--dependent O2 evolution in Chlorella saccharophila were evaluated. Addition of 4 µM AZ or 0.4 mg ml-1 DBS to photosynthesizing cells reduced the O2 evolution rate at low dissolved inorganic carbon (DIC) concentration, decreased the size of the intracellular acid-labile carbon pool, and decreased the apparent affinity of the cells for DIC. Measurement of the whole-cell affinity of cells for CO2 and HCO3- in the presence and absence of inhibitors indicated that active HCO3- transport was inhibited by AZ and DBS. The inhibition of HCO3- transport was independent of the inhibition of external and internal CA. These results suggest that the active uptake of HCO3- occurs initially by the interaction of HCO3- and a CA-like transporter.

摘要

评估了碳酸酐酶（CA）抑制剂乙酰唑胺（AZ）和葡聚糖结合磺酰胺（DBS）对嗜糖小球藻中HCO₃⁻依赖型氧气释放的影响。向进行光合作用的细胞中添加4 μM AZ或0.4 mg ml⁻¹ DBS会降低低溶解无机碳（DIC）浓度下的氧气释放速率，减小细胞内酸不稳定碳库的大小，并降低细胞对DIC的表观亲和力。在有和没有抑制剂的情况下测量细胞对CO₂和HCO₃⁻的全细胞亲和力表明，AZ和DBS抑制了活跃的HCO₃⁻转运。HCO₃⁻转运的抑制与外部和内部CA的抑制无关。这些结果表明，HCO₃⁻的主动摄取最初是通过HCO₃⁻与一种类CA转运蛋白的相互作用发生的。