Frische S, Bruno S, Fago A, Weber R E, Mozzarelli A
Danish Centre for Respiratory Adaptation, Department of Zoophysiology, University of Aarhus, 8000 Aarhus C, Denmark.
J Appl Physiol (1985). 2001 May;90(5):1679-84. doi: 10.1152/jappl.2001.90.5.1679.
Oxygen-binding properties of single red blood cells from the red-eared turtle Trachemys scripta were measured by microspectrophotometry to describe the variation in oxygen affinity of red blood cells and to gain insight into the distribution of functionally different hemoglobins among red blood cells. Methodologically, this study represents the first report on the cell-to-cell variation in oxygen-binding properties based on oxygen-binding curves of single vertebrate red blood cells. The cells differed significantly with respect to oxygen affinity. Mean oxygen pressure at half saturation of the cells in a blood sample was found to be 20.1 +/- 3.3 (SD) Torr. The distribution of oxygen affinities among red blood cells is unimodal, indicating that the two hemoglobins found in turtle blood are not segregated in distinct cells. Therefore, the functional interaction shown by these hemoglobins in vitro is likely to take place in vivo. The considerable variation in oxygen affinity between individual red blood cells calls for its incorporation in models of tissue oxygenation.
采用显微分光光度法测量了红耳龟（Trachemys scripta）单个红细胞的氧结合特性，以描述红细胞氧亲和力的变化，并深入了解功能不同的血红蛋白在红细胞中的分布情况。在方法学上，本研究是基于单个脊椎动物红细胞的氧结合曲线对细胞间氧结合特性变化的首次报道。这些细胞在氧亲和力方面存在显著差异。在一个血样中，细胞半饱和时的平均氧分压为20.1±3.3（标准差）托。红细胞中氧亲和力的分布是单峰的，这表明在龟血中发现的两种血红蛋白并非分隔在不同的细胞中。因此，这些血红蛋白在体外表现出的功能相互作用很可能在体内发生。单个红细胞之间氧亲和力的显著差异要求将其纳入组织氧合模型中。
