口腔鳞状细胞癌中CD40持续存在且极化的CD40配体表达缺失。
Maintained CD40 and loss of polarised CD40 ligand expression in oral squamous cell carcinoma.
作者信息
Loro L L, Ohlsson M, Vintermyr O K, Liavaag P G, Jonsson R, Johannessen A C
机构信息
Department of Odontology-Oral Pathology and Forensic Odontology, Haukeland University Hospital, University of Bergen, N5021 Bergen, Norway.
出版信息
Anticancer Res. 2001 Jan-Feb;21(1A):113-7.
BACKGROUND
CD40 and its ligand (CD40L) are involved in immune response and inhibition or induction of apoptosis in different tissues. Little is known about CD40 and CD40L in oral squamous cell carcinomas (OSCC).
MATERIALS AND METHODS
CD40 and CD40L were immunohistochemically evaluated in fresh-frozen samples of OSCC (n = 24) and normal oral epithelium (OE, n = 10).
RESULTS
A high proportion of OE-cells expressed CD40 (> 80%) and CD40L (> 90%) in the basal compartment compared to less than 1% CD40-positive and 1% CD40L-positive cells in the suprabasal cell layer, reflecting a zonal distribution. In well-differentiated and moderately-differentiated OSCC, there was a less pronounced zonal distribution of CD40 and a marked loss of CD40L compared to OE (p < 0.05). Poorly-differentiated OSCC maintained CD40 and markedly lost CD40L compared to OE (p < 0.05). Double immunostaining for CD40L and laminin in OE showed a basement membrane associated localisation of CD40L.
CONCLUSION
In OSCC, loss of polarised expression of CD40L and maintained expression of CD40 might be involved in tumourigenesis and immune evasion.
背景
CD40及其配体（CD40L）参与免疫反应以及不同组织中细胞凋亡的抑制或诱导。关于口腔鳞状细胞癌（OSCC）中的CD40和CD40L知之甚少。
材料与方法
采用免疫组织化学方法对24例OSCC新鲜冰冻样本和10例正常口腔上皮（OE）样本中的CD40和CD40L进行评估。
结果
与基底上层细胞层中CD40阳性细胞和CD40L阳性细胞均少于1%相比，基底细胞层中高比例的OE细胞表达CD40（>80%）和CD40L（>90%），呈现带状分布。与OE相比，高分化和中分化OSCC中CD40的带状分布不明显，且CD40L显著缺失（p<0.05）。与OE相比，低分化OSCC中CD40表达维持不变，而CD40L显著缺失（p<0.05）。OE中CD40L与层粘连蛋白的双重免疫染色显示CD40L定位于基底膜。
结论
在OSCC中，CD40L极化表达缺失和CD40表达维持不变可能参与肿瘤发生和免疫逃逸。
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