Caruso M G, Notarnicola M, Cavallini A, Di Leo A
Laboratorio di Biochimica, Institute for Digestive Disease S. de Bellis Castellana Grotte, Bari, Italy.
Anticancer Res. 2001 Jan-Feb;21(1A):429-33.
We have previously show that, 63.3% of colorectal cancers did not express the Low Density Lipoprotein Receptor (LDLR). We now report the findings of a study on the expression of LDLR and its mRNA in neoplastic tissue specimens of human colorectal cancer (CRC), carried out to verify whether the absence of the LDLR in these tumours is reflected by the absence of its transcript.
32 patients (10 females and 22 males) operated for CRC were included in the study. The LDLR levels were evaluated by immunoenzymatic assay. The LDLR-mRNA, reverse-transcribed and then amplified by polymerase chain reaction, was detected by chromatography.
The LDLR protein was present in 12 out of the 32 patients. LDLR-mRNA expression was detected in 17 out of the 32 patients. The absence of the LDLR protein was reflected by the absence of its transcript in 13 out of the 20 tumours; The LDLR mRNA levels were significantly higher in the tumours that did not expres LDLR.
The variable expression of the LDLR protein and LDLR mRNA in CRC detected in this study suggests the possibility that different therapeutic strategies may be indicated for these tumours.
我们之前已经表明，63.3%的结直肠癌不表达低密度脂蛋白受体（LDLR）。我们现在报告一项关于人结直肠癌（CRC）肿瘤组织标本中LDLR及其mRNA表达的研究结果，该研究旨在验证这些肿瘤中LDLR的缺失是否由其转录本的缺失所反映。
本研究纳入了32例接受CRC手术的患者（10名女性和22名男性）。通过免疫酶测定法评估LDLR水平。通过色谱法检测经逆转录然后通过聚合酶链反应扩增的LDLR - mRNA。
32例患者中有12例存在LDLR蛋白。32例患者中有17例检测到LDLR - mRNA表达。在20个肿瘤中的13个中，LDLR转录本的缺失反映了LDLR蛋白的缺失；在不表达LDLR的肿瘤中，LDLR mRNA水平显著更高。
本研究中检测到的CRC中LDLR蛋白和LDLR mRNA的可变表达表明，可能需要针对这些肿瘤采取不同的治疗策略。
