重组流感病毒核糖核蛋白颗粒的三维重建
Three-dimensional reconstruction of a recombinant influenza virus ribonucleoprotein particle.
作者信息
Martín-Benito J, Area E, Ortega J, Llorca O, Valpuesta J M, Carrascosa J L, Ortín J
机构信息
Centro Nacional de Biotecnología (CSIC), Campus de Cantoblanco, 28049 Madrid, Spain.
出版信息
EMBO Rep. 2001 Apr;2(4):313-7. doi: 10.1093/embo-reports/kve063.
Abstract
A three-dimensional structural model of an influenza virus ribonucleoprotein particle reconstituted in vivo from recombinant proteins and a model genomic vRNA has been generated by electron microscopy. It shows a circular shape and contains nine nucleoprotein monomers, two of which are connected with the polymerase complex. The nucleoprotein monomers show a curvature that may be responsible for the formation of helical structures in the full-size viral ribonucleoproteins. The monomers show distinct contact boundaries at the two sides of the particle, suggesting that the genomic RNA may be located in association with the nucleoprotein at the base of the ribonucleoprotein complex. Sections of the three-dimensional model show a trilobular morphology in the polymerase complex that is consistent with the presence of its three subunits.
摘要
通过电子显微镜已构建出一个在体内由重组蛋白和模型基因组 vRNA 重构的流感病毒核糖核蛋白颗粒的三维结构模型。它呈圆形，包含九个核蛋白单体，其中两个与聚合酶复合体相连。核蛋白单体呈现出一种曲率，这可能是在完整大小的病毒核糖核蛋白中形成螺旋结构的原因。单体在颗粒两侧显示出明显的接触边界，表明基因组 RNA 可能与核糖核蛋白复合体底部的核蛋白相关联。三维模型的切片显示聚合酶复合体呈三叶形形态，这与其三个亚基的存在一致。
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本文引用的文献
1
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J Virol. 2000 Feb;74(3):1307-12. doi: 10.1128/jvi.74.3.1307-1312.2000.
2
Ultrastructural and functional analyses of recombinant influenza virus ribonucleoproteins suggest dimerization of nucleoprotein during virus amplification.重组流感病毒核糖核蛋白的超微结构和功能分析表明，在病毒扩增过程中核蛋白会发生二聚化。
J Virol. 2000 Jan;74(1):156-63. doi: 10.1128/jvi.74.1.156-163.2000.
3
Replication of orthomyxoviruses.正黏病毒的复制
Adv Virus Res. 1999;54:319-48. doi: 10.1016/s0065-3527(08)60370-x.
4
Identification of amino acid residues of influenza virus nucleoprotein essential for RNA binding.鉴定流感病毒核蛋白中对RNA结合至关重要的氨基酸残基。
J Virol. 1999 Sep;73(9):7357-67. doi: 10.1128/JVI.73.9.7357-7367.1999.
5
Direct evidence that the poly(A) tail of influenza A virus mRNA is synthesized by reiterative copying of a U track in the virion RNA template.甲型流感病毒mRNA的聚腺苷酸尾是通过对病毒粒子RNA模板中的U序列进行重复拷贝而合成的直接证据。
J Virol. 1999 Apr;73(4):3473-6. doi: 10.1128/JVI.73.4.3473-3476.1999.
6
A hairpin loop at the 5' end of influenza A virus virion RNA is required for synthesis of poly(A)+ mRNA in vitro.甲型流感病毒病毒粒子RNA 5'端的发夹环是体外合成聚腺苷酸加尾mRNA所必需的。
J Virol. 1999 Mar;73(3):2109-14. doi: 10.1128/JVI.73.3.2109-2114.1999.
7
Mutational analysis of influenza A virus nucleoprotein: identification of mutations that affect RNA replication.甲型流感病毒核蛋白的突变分析：影响RNA复制的突变的鉴定
J Virol. 1999 Feb;73(2):1186-94. doi: 10.1128/JVI.73.2.1186-1194.1999.
8
Influenza virus nucleoprotein interacts with influenza virus polymerase proteins.流感病毒核蛋白与流感病毒聚合酶蛋白相互作用。
J Virol. 1998 Jul;72(7):5493-501. doi: 10.1128/JVI.72.7.5493-5501.1998.
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Ultramicroscopy. 1998 Apr;72(1-2):53-65. doi: 10.1016/s0304-3991(97)00127-7.
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J Gen Virol. 1998 Mar;79 ( Pt 3):471-8. doi: 10.1099/0022-1317-79-3-471.
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