Martín-Benito J, Area E, Ortega J, Llorca O, Valpuesta J M, Carrascosa J L, Ortín J
Centro Nacional de Biotecnología (CSIC), Campus de Cantoblanco, 28049 Madrid, Spain.
EMBO Rep. 2001 Apr;2(4):313-7. doi: 10.1093/embo-reports/kve063.
A three-dimensional structural model of an influenza virus ribonucleoprotein particle reconstituted in vivo from recombinant proteins and a model genomic vRNA has been generated by electron microscopy. It shows a circular shape and contains nine nucleoprotein monomers, two of which are connected with the polymerase complex. The nucleoprotein monomers show a curvature that may be responsible for the formation of helical structures in the full-size viral ribonucleoproteins. The monomers show distinct contact boundaries at the two sides of the particle, suggesting that the genomic RNA may be located in association with the nucleoprotein at the base of the ribonucleoprotein complex. Sections of the three-dimensional model show a trilobular morphology in the polymerase complex that is consistent with the presence of its three subunits.
通过电子显微镜已构建出一个在体内由重组蛋白和模型基因组 vRNA 重构的流感病毒核糖核蛋白颗粒的三维结构模型。它呈圆形，包含九个核蛋白单体，其中两个与聚合酶复合体相连。核蛋白单体呈现出一种曲率，这可能是在完整大小的病毒核糖核蛋白中形成螺旋结构的原因。单体在颗粒两侧显示出明显的接触边界，表明基因组 RNA 可能与核糖核蛋白复合体底部的核蛋白相关联。三维模型的切片显示聚合酶复合体呈三叶形形态，这与其三个亚基的存在一致。
