甲型流感病毒mRNA的聚腺苷酸尾是通过对病毒粒子RNA模板中的U序列进行重复拷贝而合成的直接证据。
Direct evidence that the poly(A) tail of influenza A virus mRNA is synthesized by reiterative copying of a U track in the virion RNA template.
作者信息
Poon L L, Pritlove D C, Fodor E, Brownlee G G
机构信息
Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
出版信息
J Virol. 1999 Apr;73(4):3473-6. doi: 10.1128/JVI.73.4.3473-3476.1999.
Abstract
The poly(A) tail of influenza virus mRNA is thought to be synthesized by reiterative copying of the U track near the 5' end of the virion RNA template. This has been widely accepted as a plausible hypothesis, but until now there has been no direct experimental evidence for it. Here, we report such direct evidence based on the fact that (i) replacing the U track with an A track directs synthesis of products with poly(U) tails, both in vitro and in vivo, and (ii) interrupting the U track abolishes polyadenylation in vitro.
摘要
流感病毒mRNA的聚腺苷酸尾被认为是通过对病毒粒子RNA模板5'端附近的U序列进行重复拷贝而合成的。这一观点作为一个合理的假说已被广泛接受，但迄今为止尚无直接的实验证据。在此，我们基于以下事实报告此类直接证据：（i）用A序列取代U序列可在体外和体内指导合成具有聚尿苷酸尾的产物；（ii）中断U序列会在体外消除多聚腺苷酸化。
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本文引用的文献
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