Kulås E, Ackman R G
Norsk Hydro Research Centre, P.O. Box 2560, 3907 Porsgrunn, Norway.
J Agric Food Chem. 2001 Apr;49(4):1724-9. doi: 10.1021/jf0011541.
The effectiveness of 2.32 mmol/kg (approximately 1000 ppm) of alpha-, gamma-, or delta-tocopherol (TOH), as well as different levels of alpha TOH, on the formation of hydroperoxides in fish oil was studied by monitoring the peroxide value (POV) and the formation of conjugated dienes (CD) during storage at 30 degrees C. The same order of antioxidant activity was observed by both methods. Linear regression of POV on CD showed that these data were strongly correlated (r(2) > or = 0.98). The value of the slope of the regression lines, however, differed substantially and decreased with increasing hydrogen-donating ability of the different tocopherols and with increasing alpha TOH concentration. It is suggested that this is due, at least in part, to the contribution from hydroxy compounds to the CD measurements and a greater contribution from hydroperoxy epidioxides (two peroxide groups per conjugated diene unit) to the POV than to the CD value. The degrees of formation of both these groups of oxidation products are expected to be influenced by the rate of scavenging of lipid peroxyl and alkoxyl radicals by tocopherol (alpha TOH > gamma TOH > delta TOH).
通过监测30℃储存期间的过氧化值（POV）和共轭二烯（CD）的形成，研究了2.32 mmol/kg（约1000 ppm）的α-、γ-或δ-生育酚（TOH）以及不同水平的α-TOH对鱼油中氢过氧化物形成的影响。两种方法观察到的抗氧化活性顺序相同。POV对CD的线性回归表明，这些数据高度相关（r²≥0.98）。然而，回归线斜率的值差异很大，并且随着不同生育酚供氢能力的增加以及α-TOH浓度的增加而降低。这表明，至少部分原因是羟基化合物对CD测量有贡献，而氢过氧化环氧化物（每个共轭二烯单元有两个过氧化物基团）对POV的贡献比对CD值的贡献更大。预计这两组氧化产物的形成程度会受到生育酚清除脂质过氧自由基和烷氧自由基速率的影响（α-TOH＞γ-TOH＞δ-TOH）。
