Bleesing J J, Fleisher T A
Department of Laboratory Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892-1508, USA.
Semin Hematol. 2001 Apr;38(2):169-78. doi: 10.1016/s0037-1963(01)90050-2.
Flow cytometry is increasingly used to assess the functional status of leukocytes, and practically all aspects of their life (and death) are accessible to flow cytometric study. Together with familiar features of flow cytometry, such as multiparameter immunophenotyping, cell function-based flow cytometry has provided many new insights into the relationships among lymphocyte cell surface features; intracellular processes, such as cytokine production and protein phosphorylation; and the functional status of lymphocytes in a variety of human diseases. Direct visualization and quantification of antigen-specific T cells using major histocompatibility complex (MHC)-peptide tetramer technology, in combination with functional assays, has provided the means to study specific T-cell subsets of interest. Even early in its development, this technology already has offered a better understanding of basic and clinical immunology and has invited reassessment of several long-standing immunologic concepts. Semin Hematol 38:169-178. This is a US government work. There are no restrictions on its use.
流式细胞术越来越多地用于评估白细胞的功能状态，实际上其生命（和死亡）的几乎所有方面都可通过流式细胞术研究进行检测。连同流式细胞术的常见特征，如多参数免疫表型分析，基于细胞功能的流式细胞术为淋巴细胞细胞表面特征之间的关系；细胞内过程，如细胞因子产生和蛋白质磷酸化；以及各种人类疾病中淋巴细胞的功能状态提供了许多新的见解。使用主要组织相容性复合体（MHC）-肽四聚体技术结合功能测定对抗原特异性T细胞进行直接可视化和定量，提供了研究感兴趣的特定T细胞亚群的方法。即使在其发展的早期，这项技术就已经增进了对基础和临床免疫学的理解，并促使人们重新评估一些长期存在的免疫学概念。《血液学研讨会》38:169 - 178。这是美国政府的作品。其使用不受限制。
