Martins-Sousa R, Negrão-Corrêa D, Bezerra F, Coelho P
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brasil.
Mem Inst Oswaldo Cruz. 2001 Apr;96(3):391-2. doi: 10.1590/s0074-02762001000300019.
The anesthetic effect of some water-soluble anesthesic or narcotic drugs currently used in mice was tested in molluscs of the Biomphalaria genus. Sodium thiopental was very toxic to the snails resulting in high rates of mortality in all the treatment schedules tested. Cetamine base, at concentration of 0.25 mg/ml of water, resulted in partial snail anesthesia (40% of snails were anesthetized) only after 20 h of exposition. The association of Cetamine base with Tiazine chloridrate did not improve the anesthesic effect, and higher concentrations of these drugs were toxic to the snails. Sodium pentobarbital at 0.4 mg/ml in water for 8 h was the best treatment schedule to anesthetize Biomphalaria snails. In this schedule, the snails were anesthetized without any toxic effect. The procedure provides a powerful tool for in vivo studies that demande a complete state of snail anesthesia.
目前在小鼠中使用的一些水溶性麻醉或麻醉药物的麻醉效果在双脐螺属软体动物中进行了测试。硫喷妥钠对蜗牛毒性很大，在所有测试的治疗方案中导致高死亡率。浓度为0.25mg/ml水的氯胺酮碱仅在暴露20小时后导致部分蜗牛麻醉（40%的蜗牛被麻醉）。氯胺酮碱与氯噻嗪的联合使用并没有改善麻醉效果，这些药物的更高浓度对蜗牛有毒。水中0.4mg/ml的戊巴比妥钠处理8小时是麻醉双脐螺蜗牛的最佳治疗方案。在此方案中，蜗牛被麻醉且没有任何毒性作用。该程序为需要蜗牛完全麻醉状态的体内研究提供了一个强大的工具。
