Yolcu E, Sayan B S, Yağci T, Cetin-Atalay R, Soussi T, Yurdusev N, Ozturk M
Department of Molecular Biology and Genetics and BilGen Genetics and Biotechnology Center, Bilkent University, 06533, Ankara, Turkey.
Oncogene. 2001 Mar 15;20(11):1398-401. doi: 10.1038/sj.onc.1204240.
Three monoclonal antibodies (Mabs) were generated against p53 DNA-binding core domain. When tested by immunoprecipitation, Western blot and immunofluorescence techniques, Mab 9E4, as well as 7D3 and 6B10 reacted with both wild-type and various mutant p53 proteins. The epitopes recognized by Mabs 7D3, 9E4 and 6B10 were located respectively within the amino acid residues 211-220, 281-290 and 291-300 of human p53 protein. The epitope recognized by 9E4 Mab coincides with helix 2, also called p53 DNA binding helix, which allows the direct contact of the protein with its target DNA sequences. This antibody may be useful to study transcription-dependent and transcription-independent activities of wild-type and mutant p53 proteins.
针对p53 DNA结合核心结构域产生了三种单克隆抗体（Mab）。通过免疫沉淀、蛋白质印迹和免疫荧光技术检测时，单克隆抗体9E4以及7D3和6B10与野生型和各种突变型p53蛋白均发生反应。单克隆抗体7D3、9E4和6B10识别的表位分别位于人p53蛋白的氨基酸残基211 - 220、281 - 290和291 - 300内。单克隆抗体9E4识别的表位与螺旋2重合，螺旋2也称为p53 DNA结合螺旋，它使该蛋白能够与其靶DNA序列直接接触。这种抗体可能有助于研究野生型和突变型p53蛋白的转录依赖性和非转录依赖性活性。
