Drandarevski N, Marburger A, Walther D, Reum T, Uh G, Morgenstern R
Institute of Pharmacology and Toxicology, Medical Faculty Charité, Humboldt University Berlin, Federal Republic of Germany.
J Neural Transm (Vienna). 2001;108(2):141-51. doi: 10.1007/s007020170084.
The present study was performed to investigate the influence of intrastriatal fetal mesencephalic grafts on dopaminergic mRNA expression in the non-lesioned substantia nigra pars compacta of unilaterally 6-hydroxydopamine-lesioned rats. The expression of dopamine transporter mRNA, synaptic vesicular monoamine transporter mRNA and tyrosine hydroxylase mRNA was assessed in adjacent cryostat sections using in situ hybridization. Rotational behavior induced by apomorphine and amphetamine as well as hybridization of striatal sections cut at the grafting coordinates were used to prove the functional recovery and the presence of grafted cells, respectively. After grafting, the number of rotations was decreased and hybridization signals overlying cells in the grafted striatum were detected. Mean grain densities overlying labeled neurons in the substantia nigra pars compacta of grafted rats were compared to those of shamgrafted rats and revealed differential expression of dopamine transporter mRNA, whereas synaptic vesicular monoamine transporter mRNA and tyrosine hydroxylase mRNA expression showed no difference. The results will be discussed in relation to previous in vitro and in vivo studies suggesting a reduction of functional dopamine transporter molecules in the contralateral striatum.
本研究旨在探讨纹状体内胎儿中脑移植物对单侧6-羟基多巴胺损伤大鼠未损伤黑质致密部中多巴胺能mRNA表达的影响。使用原位杂交技术在相邻的低温恒温器切片中评估多巴胺转运体mRNA、突触囊泡单胺转运体mRNA和酪氨酸羟化酶mRNA的表达。分别使用阿扑吗啡和苯丙胺诱导的旋转行为以及在移植坐标处切割的纹状体切片的杂交来证明功能恢复和移植细胞的存在。移植后，旋转次数减少，并且在移植的纹状体中检测到覆盖细胞的杂交信号。将移植大鼠黑质致密部中标记神经元上的平均颗粒密度与假移植大鼠的进行比较，结果显示多巴胺转运体mRNA存在差异表达，而突触囊泡单胺转运体mRNA和酪氨酸羟化酶mRNA表达没有差异。将结合先前的体外和体内研究结果对这些结果进行讨论，这些研究表明对侧纹状体中功能性多巴胺转运体分子减少。
