Rombout Y B, Bosch S, Mandjes B, Homan W, van der Giessen J W
National Institute of Public Health and the Environment (RIVM), Microbiological Laboratory for Health Protection, Antonie van Leeuwenhoeklaan 9, PO Box 1, 3720 BA Bilthoven, the Netherlands.
J Helminthol. 2001 Mar;75(1):67-72. doi: 10.1079/joh200034.
The genetic diversity within the genus Trichinella was studied using cleavage fragment length polymorphism (CFLP) analysis. The CFLP method generates specific fingerprints based on single nucleotide mutations. By this method the amplified intergenic regions of the 5S rRNA genes of the eight different genotypes of Trichinella were analysed. The CFLP pattern of T. spiralis was completely different compared with the sylvatic species T. britovi, T. nativa, T. nelsoni, and the genotypes Trichinella T5, Trichinella T6 and Trichinella T8. The T. pseudospiralis intergenic region can be differentiated by size from the other species of Trichinella.
利用酶切片段长度多态性（CFLP）分析研究了旋毛虫属内的遗传多样性。CFLP方法基于单核苷酸突变产生特定的指纹图谱。通过该方法分析了旋毛虫八种不同基因型5S rRNA基因的扩增基因间区域。旋毛虫的CFLP图谱与野生型物种布氏旋毛虫、本地旋毛虫、纳氏旋毛虫以及旋毛虫T5、旋毛虫T6和旋毛虫T8基因型完全不同。伪旋毛虫的基因间区域可通过大小与旋毛虫属的其他物种区分开来。
